ArticlePhotosynthesis research2014
A non-radioactive method for measuring Rubisco activase activity in the presence of variable ATP: ADP ratios, including modifications for measuring the activity and activation state of Rubisco.
Article in Photosynthesis research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Rubisco functional integrity requires a dedicated three-step maturation pathway.Science advances · 2026Article
- Advances in field-based high-throughput photosynthetic phenotyping.Journal of experimental botany · 2022Review
- Measuring Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays.Journal of experimental botany · 2020Article
- Chronic effects of microcystin-LR at environmental relevant concentrations on photosynthesis of Typha angustifolia Linn.Ecotoxicology (London, England) · 2020Article
- Overexpression of BUNDLE SHEATH DEFECTIVE 2 improves the efficiency of photosynthesis and growth in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- A single point mutation in the C-terminal extension of wheat Rubisco activase dramatically reduces ADP inhibition via enhanced ATP binding affinity.The Journal of biological chemistry · 2019Article
- A Conserved Sequence from Heat-Adapted Species Improves Rubisco Activase Thermostability in Wheat.Plant physiology · 2019Article
- Assembly-disassembly is coupled to the ATPase cycle of tobacco Rubisco activase.The Journal of biological chemistry · 2018Article
- Metabolic Reprogramming in Chloroplasts under Heat Stress in Plants.International journal of molecular sciences · 2018Review
- Whole plant chamber to examine sensitivity of cereal gas exchange to changes in evaporative demand.Plant methods · 2018Article
- Rubisco and Rubisco Activase Play an Important Role in the Biochemical Limitations of Photosynthesis in Rice, Wheat, and Maize under High Temperature and Water Deficit.Frontiers in plant science · 2017Article
- The Diverse AAA+ Machines that Repair Inhibited Rubisco Active Sites.Frontiers in molecular biosciences · 2017Review
- Metabolic and diffusional limitations of photosynthesis in fluctuating irradiance in Arabidopsis thaliana.Scientific reports · 2016Article
- Improved analysis of C4 and C3 photosynthesis via refined in vitro assays of their carbon fixation biochemistry.Journal of experimental botany · 2016Article
- Identification of potential cargo proteins of transportin protein AtTRN1 in Arabidopsis thaliana.Plant cell reports · 2016Article
- The Plastid Casein Kinase 2 Phosphorylates Rubisco Activase at the Thr-78 Site but Is Not Essential for Regulation of Rubisco Activation State.Frontiers in plant science · 2016Article
- Identification and characterization of multiple rubisco activases in chemoautotrophic bacteria.Nature communications · 2015Article
- Regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase: product inhibition, cooperativity, and magnesium activation.The Journal of biological chemistry · 2015Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes carboxylation of ribulose-1,5-bisphosphate, the first in a series of reactions leading to the incorporation of atmospheric CO₂ into biomass. Rubisco requires Rubisco activase (RCA), an AAA+ ATPase that reactivates Rubisco by remodelling the conformation of inhibitor-bound sites. RCA is regulated by the ratio of ADP:ATP, with the precise response potentiated by redox regulation of the alpha-isoform. Measuring the effects of ADP on the activation of Rubisco by RCA using the well-established photometric assay is problematic because of the adenine nucleotide requirement of 3-phosphoglycerate (3-PGA) kinase. Described here is a novel assay for measuring RCA activity in the presence of variable ratios of ADP:ATP. The assay couples the formation of 3-PGA from ribulose 1,5-bisphosphate and CO₂ to NADH oxidation through cofactor-dependent phosphoglycerate mutase, enolase, PEP carboxylase and malate dehydrogenase. The assay was used to determine the effects of Rubisco and RCA concentration and ADP:ATP ratio on RCA activity, and to measure the activation of a modified Rubisco by RCA. Variations of the basic assay were used to measure the activation state of Rubisco in leaf extracts and the activity of purified Rubisco. The assay can be automated for high-throughput processing by conducting the reactions in two stages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.