ArticleBiotechnology and bioengineering2014
Synthetic small intestinal scaffolds for improved studies of intestinal differentiation.
Article in Biotechnology and bioengineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed, 153 citations in OpenAlex.
- Biomimetic villi-crypt scaffold-on-chip with tunable mechanical properties for intestinal epithelium modeling.Materials today. Bio · 2026Article
- A New 3D Colon on a Chip to Decipher the Influence of Mechanical Forces on the Physiological Cellular Ecosystem.Advanced healthcare materials · 2026Article
- Cellular membrane protein MipA from E.coli Nissle 1917 protects against Salmonella infection.The ISME journal · 2026Article
- Application and prospects of organoid-on-a-chip in research on the intestinal mucosal barrier.Burns & trauma · 2026Review
- Gastrointestinal tract, its pathophysiology andWorld journal of gastroenterology · 2025Review
- Evaluation of Drug Permeation Enhancement by Using In Vitro and Ex Vivo Models.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Cultivating complexity: Advancements in establishing in vitro models for the mucus-adhering gut microbiota.Microbial biotechnology · 2024Review
- Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques.Materials today. Bio · 2024Review
- Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Engineered live bacteria as disease detection and diagnosis tools.Journal of biological engineering · 2023Review
- Challenges in Permeability Assessment for Oral Drug Product Development.Pharmaceutics · 2023Review
- Advancing intestinal organoid technology to decipher nano-intestine interactions and treat intestinal disease.Nano research · 2023Review
- Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022Review
- Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium.ACS biomaterials science & engineering · 2022Article
- Modeling Biochemical Gradients In Vitro to Control Cell Compartmentalization in a Microengineered 3D Model of the Intestinal Epithelium.Advanced healthcare materials · 2022Article
- Building gut from scratch - progress and update of intestinal tissue engineering.Nature reviews. Gastroenterology & hepatology · 2022Review
- Synthesis of Poly(acrylic acid)-Cysteine-Based Hydrogels with Highly Customizable Mechanical Properties for Advanced Cell Culture Applications.ACS omega · 2022Article
- Current knowledge on the multiform reconstitution of intestinal stem cell niche.World journal of stem cells · 2021Review
- Effect of substrate stiffness on human intestinal enteroids' infectivity by enteroaggregative Escherichia coli.Acta biomaterialia · 2021Article
- Imaging therapeutic peptide transport across intestinal barriers.RSC chemical biology · 2021Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In vitro intestinal models can provide new insights into small intestinal function, including cellular growth and proliferation mechanisms, drug absorption capabilities, and host-microbial interactions. These models are typically formed with cells cultured on 2D scaffolds or transwell inserts, but it is widely understood that epithelial cells cultured in 3D environments exhibit different phenotypes that are more reflective of native tissue. Our focus was to develop a porous, synthetic 3D tissue scaffold with villous features that could support the culture of epithelial cell types to mimic the natural microenvironment of the small intestine. We demonstrated that our scaffold could support the co-culture of Caco-2 cells with a mucus-producing cell line, HT29-MTX, as well as small intestinal crypts from mice for extended periods. By recreating the surface topography with accurately sized intestinal villi, we enable cellular differentiation along the villous axis in a similar manner to native intestines. In addition, we show that the biochemical microenvironments of the intestine can be further simulated via a combination of apical and basolateral feeding of intestinal cell types cultured on the 3D models.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.