ArticleNature methods2014
Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 195 papers.
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Who cites it
195 citing papers in PubMed, 371 citations in OpenAlex.
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- A paradoxical relationship between mitochondrial calcium regulation and retinal ganglion cell degeneration after axon damage.bioRxiv : the preprint server for biology · 2026Article
- Glomerulus-Specific Inhomogeneity of the Basal Activity Map in the Olfactory Bulb.International journal of molecular sciences · 2026Article
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- Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins.Biophysical journal · 2025Article
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- Intracellular CaBiologia futura · 2025Review
- Abundance-biased codon diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors.Communications biology · 2025Article
- Structure Prediction of Alternate Frame Folding Systems with AlphaFold3.Journal of chemical information and modeling · 2025Article
- Enkephalinergic Neurons in Barrington's Nucleus Gate Sex-Specific Control of Micturition.Research square · 2025Article
- The Multifaceted Role of Calcium Signaling in Regulated Necrosis.Biomolecules · 2025Review
- Regulatory Action of Calcium and Calcium Channels in Pain Pathways.International journal of biological sciences · 2025Review
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
135 more citing papers are in PubMed but not listed here.
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20 authors at 8 institutions in 2 countries.
Funding
Abstract
The quality of genetically encoded calcium indicators (GECIs) has improved dramatically in recent years, but high-performing ratiometric indicators are still rare. Here we describe a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors with a reduced number of calcium binding sites per sensor. These 'Twitch' sensors are based on the C-terminal domain of Opsanus troponin C. Their FRET responses were optimized by a large-scale functional screen in bacterial colonies, refined by a secondary screen in rat hippocampal neuron cultures. We tested the in vivo performance of the most sensitive variants in the brain and lymph nodes of mice. The sensitivity of the Twitch sensors matched that of synthetic calcium dyes and allowed visualization of tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes. Given their ratiometric readout, their brightness, large dynamic range and linear response properties, Twitch sensors represent versatile tools for neuroscience and immunology.
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