ArticleJournal of biomedical optics2014
Functional assessment of gap junctions in monolayer and three-dimensional cultures of human tendon cells using fluorescence recovery after photobleaching.
Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- hMSCs form Osteocyte-like Cell Networks within Strain-Stiffening Bottlebrush Polymer Hydrogels.bioRxiv : the preprint server for biology · 2026Article
- Connexin-43-Mediated Gap Junction Coupling Between Adipocytes Regulates Norepinephrine-Induced CaCells · 2026Article
- Interpenetrating network hydrogels for studying the role of matrix viscoelasticity in 3D osteocyte morphogenesis.Biomaterials science · 2024Article
- Connexin-Containing Vesicles for Drug Delivery.The AAPS journal · 2024Review
- Collective invasion induced by an autocrine purinergic loop through connexin-43 hemichannels.The Journal of cell biology · 2020Article
- Intercellular communication controls agonist-induced calcium oscillations independently of gap junctions in smooth muscle cells.Science advances · 2020Article
- Endothelial-Stromal Communication in Murine and Human Corneas.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Cell-cell junctions in developing and adult tendons.Tissue barriers · 2020Article
- Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Frapid: achieving full automation of FRAP for chemical probe validation.Biomedical optics express · 2016Article
- Models and methods for in vitro testing of hepatic gap junctional communication.Toxicology in vitro : an international journal published in association with BIBRA · 2015Review
- Lovastatin-Mediated Changes in Human Tendon Cells.Journal of cellular physiology · 2015Article
- Tendon mechanobiology: Current knowledge and future research opportunities.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Gap junction-mediated intercellular communication influences a variety of cellular activities. In tendons, gap junctions modulate collagen production, are involved in strain-induced cell death, and are involved in the response to mechanical stimulation. The aim of the present study was to investigate gap junction-mediated intercellular communication in healthy human tendon-derived cells using fluorescence recovery after photobleaching (FRAP). The FRAP is a noninvasive technique that allows quantitative measurement of gap junction function in living cells. It is based on diffusion-dependent redistribution of a gap junction-permeable fluorescent dye. Using FRAP, we showed that human tenocytes form functional gap junctions in monolayer and three-dimensional (3-D) collagen I culture. Fluorescently labeled tenocytes following photobleaching rapidly reacquired the fluorescent dye from neighboring cells, while HeLa cells, which do not communicate by gap junctions, remained bleached. Furthermore, both 18 β-glycyrrhetinic acid and carbenoxolone, standard inhibitors of gap junction activity, impaired fluorescence recovery in tendon cells. In both monolayer and 3-D cultures, intercellular communication in isolated cells was significantly decreased when compared with cells forming many cell-to-cell contacts. In this study, we used FRAP as a tool to quantify and experimentally manipulate the function of gap junctions in human tenocytes in both two-dimensional (2-D) and 3-D cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.