ReviewProtoplasma2014
FRET-FLIM applications in plant systems.
Review in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches.Plant methods · 2026Review
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- Cellular demiurgs - engineer to understand.Protoplasma · 2026Article
- A quantitative split firefly luciferase complementation assay (SplitLUC) for in planta protein-protein interactions.Protoplasma · 2026Article
- Advances and opportunities for computational interrogation of plant proteins.The Plant journal : for cell and molecular biology · 2026Review
- Cellular view of metabolism: metabolic biomolecular condensates.The New phytologist · 2025Review
- Systemic and Phloem-Specific Protein Targeting by High Affinity Nanobodies Expressed From a Plant RNA Virus Vector.Molecular plant pathology · 2025Article
- Optimization of FRET imaging in Arabidopsis protoplasts.Molecules and cells · 2025Article
- Drought-dependent regulation of cell coupling in Arabidopsis leaf epidermis requires plasmodesmal protein NHL12.Journal of experimental botany · 2024Article
- Transmembrane proteins in grape immunity: current knowledge and methodological advances.Frontiers in plant science · 2024Review
- 2 in 1 Vectors Improve in Planta BiFC and FRET Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Phase separation-based visualization of protein-protein interactions and kinase activities in plants.The Plant cell · 2023Article
- One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells.Plant methods · 2023Article
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- Protein-Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Forster Resonance Energy Transfer (FRET) to Visualize Protein-Protein Interactions in the Plant Cell.Methods in molecular biology (Clifton, N.J.) · 2023Article
- FRET-FLIM to Determine Protein Interactions and Membrane Topology of Enzyme Complexes.Current protocols · 2022Article
- Visualization of in vivo protein-protein interactions in plants.Journal of experimental botany · 2022Review
- Tracking of fluorescent antibiotic conjugate in planta utilizing fluorescence lifetime imaging.Planta · 2021Article
- Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis.Journal of visualized experiments : JoVE · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hallmark of cellular processes is the spatio-temporally regulated interplay of biochemical components. Assessing spatial information of molecular interactions within living cells is difficult using traditional biochemical methods. Developments in green fluorescent protein technology in combination with advances in fluorescence microscopy have revolutionised this field of research by providing the genetic tools to investigate the spatio-temporal dynamics of biomolecules in live cells. In particular, fluorescence lifetime imaging microscopy (FLIM) has become an inevitable technique for spatially resolving cellular processes and physical interactions of cellular components in real time based on the detection of Förster resonance energy transfer (FRET). In this review, we provide a theoretical background of FLIM as well as FRET-FLIM analysis. Furthermore, we show two cases in which advanced microscopy applications revealed many new insights of cellular processes in living plant cells as well as in whole plants.
Indexed as
Identifiers
24390247What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.