ArticleMolecular and biochemical parasitology2014
A map of 5-methylcytosine residues in Trypanosoma brucei tRNA revealed by sodium bisulfite sequencing.
Article in Molecular and biochemical parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Nanopore direct RNA sequencing and the epitranscriptome: Advances in mapping native RNA landscapes.iMeta · 2026Review
- TheMicrobiology resource announcements · 2025Article
- [Advances in mapping analysis of ribonucleic acid modifications through sequencing].Se pu = Chinese journal of chromatography · 2024Review
- A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing.PloS one · 2024Article
- RNA Methylation in ncRNA: Classes, Detection, and Molecular Associations.Frontiers in genetics · 2018Article
- The RNA methyltransferase Dnmt2 methylates DNA in the structural context of a tRNA.RNA biology · 2017Article
- Detecting RNA modifications in the epitranscriptome: predict and validate.Nature reviews. Genetics · 2017Review
- Chemical and Conformational Diversity of Modified Nucleosides Affects tRNA Structure and Function.Biomolecules · 2017Review
- Base pairing and structural insights into the 5-formylcytosine in RNA duplex.Nucleic acids research · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In protozoan parasites, there is little information on the presence of covalent RNA modifications which comprise the epitranscriptome. Therefore, we determined if T. brucei tRNA(Asp(GUC)), tRNA(Gly(GCC)), tRNA(Val(AAC)), and tRNA(Tyr(GUA)) contain 5-methylcytosines via RNA bisulfite sequencing. Most tRNAs examined have at least one 5-methylcytosine at the variable region-TψC junction. Only tRNA(Gly(GCC)) displayed methylation of C40 in the anticodon stem, and there was partial methylation at this site. There is no evidence for methylation of C38 in the anticodon loop in the tRNAs analyzed. Analysis of tRNA(Tyr(GUA)) demonstrates that both unspliced and spliced molecules contain C48 methylation, indicating tRNA cytosine methylation can precede tRNA splicing. Overall, our data indicate that T. brucei tRNAs contain 5-methylcytosine residues in some, but potentially not all standard eukaryotic positions. The levels of cytosine methylation of different T. brucei tRNAs vary, suggesting the presence of a mechanism for methylation control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.