ArticleChemistry & biology2014
Highly efficient self-replicating RNA enzymes.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Fuel-Driven Catalytic Molecular Templating.Journal of the American Chemical Society · 2026Article
- Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Exploring the space of self-reproducing ribozymes using generative models.Nature communications · 2025Article
- Information propagation through enzyme-free catalytic templating of DNA dimerization with weak product inhibition.Nature chemistry · 2025Article
- Cross-chiral exponential amplification of an RNA enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A cis-acting ligase ribozyme generates circular RNA in vitro for ectopic protein functioning.Nature communications · 2024Article
- Building Synthetic Cells─From the Technology Infrastructure to Cellular Entities.ACS synthetic biology · 2024Review
- Evolution at the Origins of Life?Life (Basel, Switzerland) · 2024Review
- Minimal RNA self-reproduction discovered from a random pool of oligomers.Chemical science · 2023Article
- RNA ligase ribozymes with a small catalytic core.Scientific reports · 2023Article
- Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations.Nature communications · 2023Article
- Experimental exploration of a ribozyme neutral network using evolutionary algorithm and deep learning.Nature communications · 2022Article
- Template-Free Assembly of Functional RNAs by Loop-Closing Ligation.Journal of the American Chemical Society · 2022Article
- Heat flows in rock cracks naturally optimize salt compositions for ribozymes.Nature chemistry · 2021Article
- Self-Sorting in Dynamic Combinatorial Libraries Leads to the Co-Existence of Foldamers and Self-Replicators.Angewandte Chemie (International ed. in English) · 2021Article
- Chemical Fueling Enables Molecular Complexification of Self-Replicators*.Angewandte Chemie (International ed. in English) · 2021Article
- tRNA sequences can assemble into a replicator.eLife · 2021Article
- On the definition of a self-sustaining chemical reaction system and its role in heredity.Biology direct · 2020Article
- Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation.Journal of the American Chemical Society · 2020Article
- Template-Directed Copying of RNA by Non-enzymatic Ligation.Angewandte Chemie (International ed. in English) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
An RNA enzyme has been developed that catalyzes the joining of oligonucleotide substrates to form additional copies of itself, undergoing self-replication with exponential growth. The enzyme also can cross-replicate with a partner enzyme, resulting in their mutual exponential growth and enabling self-sustained Darwinian evolution. The opportunity for inventive evolution within this synthetic genetic system depends on the diversity of the evolving population, which is limited by the catalytic efficiency of the enzyme. Directed evolution was used to improve the efficiency of the enzyme and increase its exponential growth rate to 0.14 min(-1), corresponding to a doubling time of 5 min. This is close to the limit of 0.21 min(-1) imposed by the rate of product release, but sufficient to enable more than 80 logs of growth per day.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.