ArticleThe international journal of biochemistry & cell biology2014
Osteoblast differentiation and migration are regulated by dynamin GTPase activity.
Article in The international journal of biochemistry & cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
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- Circ_0003764 Regulates the Osteogenic Differentiation of Periodontal Ligament Stem Cells.International dental journal · 2024Article
- Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling.eLife · 2024Article
- Protein tyrosine phosphatases in skeletal development and diseases.Bone research · 2022Review
- Synthesis of Phthaladyn-29 and Naphthalimide-10, GTP Site Directed Dynamin GTPase Inhibitors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Treadmill exercise influences the microRNA profiles in the bone tissues of mice.Experimental and therapeutic medicine · 2021Article
- Effects of Premating Calcium and Phosphorus Supplementation on Reproduction Efficiency of Grazing Yak Heifers.Animals : an open access journal from MDPI · 2021Article
- Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury.Kidney diseases (Basel, Switzerland) · 2020Article
- Hyperglycemia impairs osteoblast cell migration and chemotaxis due to a decrease in mitochondrial biogenesis.Molecular and cellular biochemistry · 2020Article
- Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis.The Korean journal of parasitology · 2020Article
- [Effect of knocking down Piezo1 mechanically sensitive protein on migration of MC3T3-E1 osteoblast cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019Article
- Mesenchymal Stem Cell Migration during Bone Formation and Bone Diseases Therapy.International journal of molecular sciences · 2018Review
- Simulated microgravity induces a cellular regression of the mature phenotype inCell death discovery · 2018Article
- SRC kinase inhibition with saracatinib limits the development of osteolytic bone disease in multiple myeloma.Oncotarget · 2016Article
- Nck influences preosteoblastic/osteoblastic migration and bone mass.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy: chemical-physical properties and human pulp cells response.Clinical oral investigations · 2015Article
- Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration.Cell research · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bone formation is controlled by osteoblasts, but the signaling proteins that control osteoblast differentiation and function are still unclear. We examined if the dynamin GTPase, which is associated with actin remodeling and migration in other cells, plays a role in osteoblast differentiation and migration. Dynamin mRNA was expressed in primary osteoblasts throughout differentiation (0-21 days). However, alkaline phosphatase (ALP) activity, a marker of osteoblast differentiation, was decreased in osteoblasts over-expressing dynamin. Conversely, ALP activity was increased following shRNA-mediated knockdown of dynamin and in osteoblasts treated with the dynamin inhibitor, dynasore. Dynasore also reduced c-fos and osterix expression, markers of early osteoblasts, suggesting a role for dynamin in pre-osteoblast to osteoblast differentiation. Since dynamin GTPase activity is regulated by tyrosine phosphorylation, we examined the mechanism of dynamin dephosphorylation in osteoblasts. Dynamin formed a protein complex with the tyrosine phosphatase PTP-PEST and inhibition of phosphatase activity increased the level of phosphorylated dynamin. Further, PTP-PEST blocked the Src-mediated increase in the phosphorylation and GTPase activity of wild-type dynamin but not the phosphorylation mutant dynY231F/Y597F. Although ALP activity was increased in osteoblasts expressing GTPase-defective dynK44A, and to a lesser extent dynY231F/Y597F, osteoblast migration was significantly inhibited by dynK44A and dynY231F/Y597F. These studies demonstrate a novel role for dynamin GTPase activity and phosphorylation in osteoblast differentiation and migration, which may be important for bone formation.
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