ArticleMicrobial cell factories2014
Isolation of a novel alkaline-stable lipase from a metagenomic library and its specific application for milkfat flavor production.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 338 citations in OpenAlex.
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- Screening of Marine Bacteria for Lipase Activity and Application as Whole-Cell Biocatalysts.Microorganisms · 2026Article
- Metabolic Responses ofACS omega · 2026Article
- Characterization and Enzymatic Potential of Bacteria and Fungi From Mwakirunge Dumpsite, Kenya.International journal of microbiology · 2025Article
- Tuning fungal promoters for the expression of eukaryotic proteins.World journal of microbiology & biotechnology · 2024Review
- Characterizing lipid constituents of B. moojeni snake venom: a comparative approach for chemical and biological investigations.Archives of toxicology · 2024Article
- Marine-Derived Lipases for Enhancing Enrichment of Very-Long-Chain Polyunsaturated Fatty Acids with Reference to Omega-3 Fatty Acids.Marine drugs · 2024Review
- Bacillus sp.: A Remarkable Source of Bioactive Lipopeptides.Advances in biochemical engineering/biotechnology · 2022Article
- Optimization of a novel lipid extraction process from microalgae.Scientific reports · 2021Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- Microbial lipases and their industrial applications: a comprehensive review.Microbial cell factories · 2020Review
- Acceleration of Swiss cheese ripening by microbial lipase without affecting its quality characteristics.Journal of food science and technology · 2019Article
- Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library.Frontiers in microbiology · 2019Article
- Harnessing Marine Biocatalytic Reservoirs for Green Chemistry Applications through Metagenomic Technologies.Marine drugs · 2018Review
- Characterization of Uncultured Genome Fragment from Soil Metagenomic Library Exposed Rare Mismatch of Internal Tetranucleotide Frequency.Frontiers in microbiology · 2016Article
- Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications.International journal of molecular sciences · 2015Review
- Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris.Microbial cell factories · 2015Article
- Est16, a New Esterase Isolated from a Metagenomic Library of a Microbial Consortium Specializing in Diesel Oil Degradation.PloS one · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipolytic enzymes are commonly used to produce desired flavors in lipolyzed milkfat (LMF) manufacturing processes. However, the choice of enzyme is critical because it determines the final profile of fatty acids released and the consequent flavor of the product. We previously constructed a metagenomic library from marine sediments, to explore the novel enzymes which have unique properties useful in flavor-enhancing LMF.
resultsA novel lipase Est_p6 was isolated from a metagenomic library and was expressed highly in E.coli. Bioinformatic analysis indicated that Est_p6 belongs to lipolytic enzyme family IV, the molecular weight of purified Est_p6 was estimated at 36 kDa by SDS-PAGE. The hydrolytic activity of the enzyme was stable under alkaline condition and the optimal temperature was 50°C. It had a high specific activity (2500 U/mg) toward pNP butyrate (pNP-C4), with K(m) and V(max) values of 1.148 mM and 3497 μmol∙min⁻¹∙mg⁻¹, respectively. The enzyme activity was enhanced by DTT and was not significantly inhibited by PMSF, EDTA or SDS. This enzyme also showed high hydrolysis specificity for myristate (C14) and palmitate (C16). It seems that Est_p6 has safety for commercial LMF flavor production and food manufacturing processes.
conclusionsThe ocean is a vast and largely unexplored resource for enzymes. According the outstanding alkaline-stability of Est_p6 and it produced myristic acid and palmitic acid more efficiently than other free fatty acids in lipolyzed milkfat. This novel lipase may be used to impart a distinctive and desirable flavor and odor in milkfat flavor production.
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