ArticleLipids in health and disease2014
The influence of EPA and DHA on markers of inflammation in 3T3-L1 cells at different stages of cellular maturation.
Article in Lipids in health and disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Reduction of circulating FABP4 level by treatment with omega-3 fatty acid ethyl esters.Lipids in health and disease · 2016Trial
- Comparison of Serum and Cervical Mucus Docosahexaenoic Acid (DHA) Levels in Patients with Polycystic Ovary Syndrome and Healthy Controls.Journal of clinical medicine · 2025Article
- Review
- Serum fatty acid profiles associated with metabolic risk in women with polycystic ovary syndrome.Frontiers in endocrinology · 2023Article
- Docosahexaenoic Acid Counteracts the Hypoxic-Induced Inflammatory and Metabolic Alterations in 3T3-L1 Adipocytes.Nutrients · 2022Article
- Docosahexaenoic Acid Delivery Systems, Bioavailability, Functionality, and Applications: A Review.Foods (Basel, Switzerland) · 2022Review
- Interleukin-6 mimics insulin-dependent cellular distribution of some cytoskeletal proteins and Glut4 transporter without effect on glucose uptake in 3T3-L1 adipocytes.Histochemistry and cell biology · 2022Article
- Valorization of Side Stream Products from Sea Cage Fattened Bluefin Tuna (Marine drugs · 2022Article
- Metabolism in Retinopathy of Prematurity.Life (Basel, Switzerland) · 2021Review
- Maternal Supplementation with Polyphenols and Omega-3 Fatty Acids during Pregnancy: Prenatal Effects on Growth and Metabolism.Animals : an open access journal from MDPI · 2021Article
- Maternal Supplementation with Polyphenols and Omega-3 Fatty Acids during Pregnancy: Effects on Growth, Metabolism, and Body Composition of the Offspring.Animals : an open access journal from MDPI · 2020Article
- DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment.International journal of molecular sciences · 2020Article
- Short-Term Responses to Fatty Acids on Lipid Metabolism and Adipogenesis in Rainbow Trout (International journal of molecular sciences · 2020Article
- Concurrent EPA and DHA Supplementation Impairs Brown Adipogenesis of C2C12 Cells.Frontiers in genetics · 2020Article
- Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice.Scientific reports · 2019Article
- Comparison of Fatty Acid and Gene Profiles in Skeletal Muscle in Normal and Obese C57BL/6J Mice before and after Blunt Muscle Injury.Frontiers in physiology · 2018Article
- The pro-/anti-inflammatory effects of different fatty acids on visceral adipocytes are partially mediated by GPR120.European journal of nutrition · 2017Article
- DHA increases adiponectin expression more effectively than EPA at relative low concentrations by regulating PPARγ and its phosphorylation at Ser273 in 3T3-L1 adipocytes.Nutrition & metabolism · 2017Article
- Review: adiponectin in retinopathy.Biochimica et biophysica acta · 2016Review
- Targeting inflammation in metabolic syndrome.Translational research : the journal of laboratory and clinical medicine · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEPA and DHA have been reported to have anti-obesity and anti-inflammatory properties. Recent studies revealed that these positive actions of n-3 PUFA at least partially are connected with their influence on metabolism and secretory functions of the adipose tissue. However, their impact on old adipocytes is still poorly understood. Therefore the aim of the present study was to evaluate the influence of EPA and DHA on markers of inflammation in 3T3-L1 cells at different stages of cellular maturation.
methodsYoung, mature and old differentiated 3T3-L1 adipocytes were cultured for 48 h in the presence of 100 μM EPA, or 50 μM DHA complexed to albumin, whereas in control conditions only albumin was added to the medium. The Oil Red O staining was used to confirm adipocytes differentiation, and measure triglycerides content in cells. The concentration of adipokines (interleukin 6, adiponectin and leptin) in conditioned media was measured using mouse-specific ELISA kits.
resultsThe fat accumulation in 3T3-L1 adipocytes was positively correlated with their age; however, EPA and DHA did not affect lipid accumulation on any stage of maturation. EPA and DHA increased the concentration of secreted adiponectin when compared with control, but only in the case of young adipocytes (58% and 35%, respectively). Moreover, EPA supplementation increased interleukin 6 concentration in conditioned medium, while DHA exerted an opposite effect on all stages of cellular maturation. Furthermore, EPA treatment increased leptin release from young cells, while DHA did not affect the secretion of this adipokine. In mature 3T3-L1 adipocytes both experimental factors decreased synthesis of leptin; however, in old cells no impact of these PUFA was noted.
conclusionsIn summary, age is an important determinant of fat accumulation in adipocytes and affects adipokines secretion by these cells. Moreover, the impact of investigated fatty acids: EPA and DHA on fat cells varies depending on the stage of maturation, and seems to be stronger in young cells than in mature and old ones. Docosahexaenoic acid exerts an anti-inflammatory action; however, on the basis of the obtained data it was not possible to determine whether eicosapentaenoic acid shows anti- or pro-inflammatory properties.
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