ArticleThe Journal of eukaryotic microbiology
The acidocalcisome vacuolar transporter chaperone 4 catalyzes the synthesis of polyphosphate in insect-stages of Trypanosoma brucei and T. cruzi.
Article in The Journal of eukaryotic microbiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Novel binding partners of the Vacuolar Transporter Chaperone (VTC) complex in Acidocalcisomes of Leishmania tarentolae.PLoS neglected tropical diseases · 2026Article
- Article
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- Inorganic Polyphosphate Modulates Chromosome Transmission Fidelity in the Fission YeastBiomolecules · 2025Article
- Review
- A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.Plant molecular biology · 2024Article
- A mammalian model reveals inorganic polyphosphate channeling into the nucleolus and induction of a hyper-condensate state.Cell reports methods · 2024Article
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- A spatially resolved elemental nanodomain organization within acidocalcisomes inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- Biomimetic Polyphosphate Materials: Toward Application in Regenerative Medicine.Progress in molecular and subcellular biology · 2022Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- The IPBiochimica et biophysica acta. Molecular cell research · 2021Review
- Metabolic control of acclimation to nutrient deprivation dependent on polyphosphate synthesis.Science advances · 2020Article
- Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae.Frontiers in plant science · 2020Review
- 5-Diphosphoinositol pentakisphosphate (5-IPThe Journal of biological chemistry · 2018Article
- Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids.Molecular microbiology · 2018Article
- Importance of polyphosphate in theMicrobial cell (Graz, Austria) · 2018Article
- The inositol pyrophosphate synthesis pathway in Trypanosoma brucei is linked to polyphosphate synthesis in acidocalcisomes.Molecular microbiology · 2017Article
- Acidocalcisomes of eukaryotes.Current opinion in cell biology · 2016Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Polyphosphate is a polymer of inorganic phosphate found in both prokaryotes and eukaryotes. Polyphosphate typically accumulates in acidic, calcium-rich organelles known as acidocalcisomes, and recent research demonstrated that vacuolar transporter chaperone 4 catalyzes its synthesis in yeast. The human pathogens Trypanosoma brucei and T. cruzi possess vacuolar transporter chaperone 4 homologs. We demonstrate that T. cruzi vacuolar transporter chaperone 4 localizes to acidocalcisomes of epimastigotes by immunofluorescence and immuno-electron microscopy and that the recombinant catalytic region of the T. cruzi enzyme is a polyphosphate kinase. RNA interference of the T. brucei enzyme in procyclic form parasites reduced short chain polyphosphate levels and resulted in accumulation of pyrophosphate. These results suggest that this trypanosome enzyme is an important component of a polyphosphate synthase complex that utilizes ATP to synthesize and translocate polyphosphate to acidocalcisomes in insect stages of these parasites.
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