ArticlePloS one2013
Comparative proteomics profile of lipid-cumulating oleaginous yeast: an iTRAQ-coupled 2-D LC-MS/MS analysis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Isolation and Pathogenicity of a Natural Recombinant Pig Reproductive and Respiratory Syndrome Virus in Northeast China.Viruses · 2025Article
- S-Nitrosylated Proteins Involved in Autophagy inLife (Basel, Switzerland) · 2023Article
- Isolation and proteomic study of fish liver lipid droplets.Biophysics reports · 2023Article
- Antihypertensive, antidyslipidemic, and renoprotective effects ofIranian journal of basic medical sciences · 2023Article
- Response of Saccharomyces cerevisiae W303 to Iron and Lead Toxicity in Overloaded Conditions.Current microbiology · 2021Article
- Omics Approaches to Study Formation and Function of Human Placental Syncytiotrophoblast.Frontiers in cell and developmental biology · 2021Review
- Article
- Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond.FEMS yeast research · 2020Review
- Optimization of protein isolation by proteomic qualification from Cutaneotrichosporon oleaginosus.Analytical and bioanalytical chemistry · 2020Article
- Proteome analysis of xylose metabolism inBiotechnology for biofuels · 2019Article
- Exploitation of genus Rhodosporidium for microbial lipid production.World journal of microbiology & biotechnology · 2017Review
- Integrative transcriptomic and proteomic analysis of the mutant lignocellulosic hydrolyzate-tolerantEngineering in life sciences · 2017Article
- Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals.Frontiers in microbiology · 2017Review
- Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.PloS one · 2016Article
- The prion protein family: a view from the placenta.Frontiers in cell and developmental biology · 2014Review
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Authors and funding
4 authors.
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Abstract
Accumulation of intracellular lipid in oleaginous yeast cells has been studied for providing an alternative supply for energy, biofuel. Numerous studies have been conducted on increasing lipid content in oleaginous yeasts. However, few explore the mechanism of the high lipid accumulation ability of oleaginous yeast strains at the proteomics level. In this study, a time-course comparative proteomics analysis was introduced to compare the non-oleaginous yeast Saccharomyces cerevisiae, with two oleaginous yeast strains, Cryptococcus albidus and Rhodosporidium toruloides at different lipid accumulation stages. Two dimensional LC-MS/MS approach has been applied for protein profiling together with isobaric tag for relative and absolute quantitation (iTRAQ) labelling method. 132 proteins were identified when three yeast strains were all at early lipid accumulation stage; 122 and 116 proteins were found respectively within cells of three strains collected at middle and late lipid accumulation stages. Significantly up-regulation or down-regulation of proteins were experienced among comparison. Essential proteins correlated to lipid synthesis and regulation were detected. Our approach provides valuable indication and better understanding for lipid accumulation mechanism from proteomics level and would further contribute to genetic engineering of oleaginous yeasts.
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