Trial reportPloS one2013
Comparison of the bacterial composition and structure in symptomatic and asymptomatic endodontic infections associated with root-filled teeth using pyrosequencing.
Trial report in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Association between endodontic symptoms and root canal microbiota: a systematic review and meta-analysis of bacteroidetes, spirochaetes and fusobacteriales.Clinical oral investigations · 2024Pooled it
- Laboratory investigation of intra-tooth variability in microbial communities from clinically isolated root canal and filling material.Clinical oral investigations · 2026Article
- Targeted detection and antimicrobial susceptibility of common pathogens of failed root canal treatments: A cross-sectional study.Journal of conservative dentistry and endodontics · 2026Article
- Profiling of microbes associated with chronic irreversible pulpitis using metagenomic next-generation sequencing.BMC oral health · 2025Article
- Exploring Relationships Within the Microbiome of Root Canal Infections and the Influence of Associated Clinical Parameters.International endodontic journal · 2025Article
- A polymicrobial perspective into the ecological role of Enterococcus faecalis in dental root canal infections.NPJ biofilms and microbiomes · 2025Review
- Relationship of MMP-9 with the clinical course of apical periodontitis and the main bacterial species in the oral microbiota.Odontology · 2024Article
- Linking periodontal pathogens with endoperio lesions.Bioinformation · 2024Article
- Taxonomic abundance in primary and secondary root canal infections.International endodontic journal · 2023Article
- Multispecies biofilm removal by a multisonic irrigation system in mandibular molars.International endodontic journal · 2022Article
- Improving the microbial sampling and analysis of secondary infected root canals by passive ultrasonic irrigation.Clinical oral investigations · 2022Article
- Molecular characterization and antibacterial activity of oral antibiotics and copper nanoparticles against endodontic pathogens commonly related to health care-associated infections.Clinical oral investigations · 2021Article
- Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study.BMC oral health · 2021Article
- Subinhibitory Antibiotic Concentrations Enhance Biofilm Formation of ClinicalAntibiotics (Basel, Switzerland) · 2021Article
- Microbiological Aspects of Root Canal Infections and Disinfection Strategies: An Update Review on the Current Knowledge and Challenges.Frontiers in oral health · 2021Review
- New Strategy of Reducing Biofilm Forming Bacteria in Oral Cavity by Bismuth Nanoparticles.BioMed research international · 2021Article
- Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence.Journal of clinical medicine · 2020Article
- Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells.Scientific reports · 2020Article
- A Pilot Study of Chronological Microbiota Changes in a Rat Apical Periodontitis Model.Microorganisms · 2020Article
- Prevalence of the GenusJournal of clinical medicine · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Residual microorganisms and/or re-infections are a major cause for root canal therapy failure. Understanding of the bacterial content could improve treatment protocols. Fifty samples from 25 symptomatic and 25 asymptomatic previously root-filled teeth were collected from Sudanese patients with periradicular lesions. Amplified 16S rRNA gene (V1-V2) variable regions were subjected to pyrosequencing (FLX 454) to determine the bacterial profile. Obtained quality-controlled sequences from forty samples were classified into 741 operational taxonomic units (OTUs) at 3% dissimilarity, 525 at 5% dissimilarity and 297 at 10% dissimilarity, approximately corresponding to species-, genus- and class levels. The most abundant phyla were: Firmicutes (29.9%), Proteobacteria (26.1%), Actinobacteria (22.72%), Bacteroidetes (13.31%) and Fusobacteria (4.55%). Symptomatic patients had more Firmicutes and Fusobacteria than asymptomatic patients, while asymptomatic patients showed more Proteobacteria and Actinobacteria. Interaction of disease status and age was observed by two-way ANOSIM. Canonical correspondence analysis for age, tooth restoration and disease status showed a correlation of disease status with the composition and prevalence of different members of the microbial community. The pyrosequencing analysis revealed a distinctly higher diversity of the microbiota compared to earlier reports. The comparison of symptomatic and asymptomatic patients showed a clear association of the composition of the bacterial community with the presence and absence of symptoms in conjunction with the patients' age.
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