ArticlePloS one2013
A novel role for an ECF sigma factor in fatty acid biosynthesis and membrane fluidity in Pseudomonas aeruginosa.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Redox protection by bacterial glutathione peroxidase drives virulence inRedox report : communications in free radical research · 2026Article
- Mechanisms of thermoregulation inJournal of bacteriology · 2026Review
- Stigmurin derivatives as potent-biofilm eradicating agents against the major human opportunistic pathogenFrontiers in microbiology · 2026Article
- The Genetic Background and Culture Medium Only Marginally Affect the In Vitro Evolution ofAntibiotics (Basel, Switzerland) · 2025Article
- Squalamine and claramine A1 disperseBiofilm · 2025Article
- Bacteria detect neutrophils via a system that responds to hypochlorous acid and flow.bioRxiv : the preprint server for biology · 2025Article
- Review
- Article
- Membrane fluidity homeostasis is required for tobramycin-enhanced biofilm inMicrobiology spectrum · 2024Article
- Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress.PloS one · 2024Article
- High affinity iron uptake by pyoverdine in Pseudomonas aeruginosa involves multiple regulators besides Fur, PvdS, and FpvI.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- Rewiring the specificity of extracytoplasmic function sigma factors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- TacklingBiomolecules · 2020Article
- Activation of the Cell Wall Stress Response inMicroorganisms · 2020Article
- Membrane-Interactive Compounds FromFrontiers in microbiology · 2020Article
- The Temperature-Regulation ofFrontiers in microbiology · 2020Article
- Microfluidic-based transcriptomics reveal force-independent bacterial rheosensing.Nature microbiology · 2019Article
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa.Scientific reports · 2018Article
- Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide onPathogens (Basel, Switzerland) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracytoplasmic function (ECF) sigma factors are members of cell-surface signaling systems, abundant in the opportunistic pathogen Pseudomonas aeruginosa. Twenty genes coding for ECF sigma factors are present in P. aeruginosa sequenced genomes, most of them being part of TonB systems related to iron uptake. In this work, poorly characterized sigma factors were overexpressed in strain PA14, in an attempt to understand their role in the bacterium's physiology. Cultures overexpressing SigX displayed a biphasic growth curve, reaching stationary phase earlier than the control strain, followed by subsequent growth resumption. During the first stationary phase, most cells swell and die, but the remaining cells return to the wild type morphology and proceed to a second exponential growth. This is not due to compensatory mutations, since cells recovered from late time points and diluted into fresh medium repeated this behavior. Swollen cells have a more fluid membrane and contain higher amounts of shorter chain fatty acids. A proteomic analysis was performed to identify differentially expressed proteins due to overexpression of sigX, revealing the induction of several fatty acid synthesis (FAS) enzymes. Using qRT-PCR, we showed that at least one isoform from each of the FAS pathway enzymes were upregulated at the mRNA level in the SigX overexpressing strain thus pointing to a role for this ECF sigma factor in the FAS regulation in P. aeruginosa.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.