ArticlePloS one2013
Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Sequence and structure of protein binding sites in RNA impact biomolecular condensates.Molecular biology of the cell · 2026Article
- Sequence and structure of protein binding sites in RNA impact biomolecular condensates.bioRxiv : the preprint server for biology · 2026Article
- The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progression.Nucleic acids research · 2026Article
- Targeting APEX2 to the mRNA encoding fatty acid synthase β in yeast identifies interacting proteins that control its abundance in the cell cycle.Molecular biology of the cell · 2023Article
- Hyperactive Ras disrupts cell size control and a key step in cell cycle entry in budding yeast.Genetics · 2023Article
- Polysome propensity and tunable thresholds in coding sequence length enable differential mRNA stability.Science advances · 2023Article
- Septin Defects Favour Symmetric Inheritance of the Budding Yeast Deceptive Courtship Memory.International journal of molecular sciences · 2023Article
- mRNA-binding proteins and cell cycle progression.Trends in genetics : TIG · 2022Article
- Article
- Stress granules display bistable dynamics modulated by Cdk.The Journal of cell biology · 2021Article
- Review
- Cell-size regulation in budding yeast does not depend on linear accumulation of Whi5.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast.Molecular cell · 2020Article
- Dissimilation of synonymous codon usage bias in virus-host coevolution due to translational selection.Nature ecology & evolution · 2020Article
- A secretion-enhancing cis regulatory targeting element (SECReTE) involved in mRNA localization and protein synthesis.PLoS genetics · 2019Article
- Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.Cell cycle (Georgetown, Tex.) · 2019Article
- Translation elongation and mRNA stability are coupled through the ribosomal A-site.RNA (New York, N.Y.) · 2018Article
- Translational Control by Prion-like Proteins.Trends in cell biology · 2018Review
- PTRE-seq reveals mechanism and interactions of RNA binding proteins and miRNAs.Nature communications · 2018Article
- GWIPS-viz: 2018 update.Nucleic acids research · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Whi3 is an RNA binding protein known to bind the mRNA of the yeast G1 cyclin gene CLN3. It inhibits CLN3 function, but the mechanism of this inhibition is unclear; in previous studies, Whi3 made no observable difference to CLN3 mRNA levels, translation, or protein abundance. Here, we re-approach this issue using microarrays, RNA-Seq, ribosome profiling, and other methods. By multiple methods, we find that the whi3 mutation causes a small but consistent increase in the abundance of hundreds of mRNAs, including the CLN3 mRNA. The effect on various mRNAs is roughly in proportion to the density of GCAU or UGCAU motifs carried by these mRNAs, which may be a binding site for Whi3. mRNA instability of Whi3 targets may in part depend on a 3' AU rich element (ARE), AUUUUA. In addition, the whi3 mutation causes a small increase in the translational efficiency of CLN3 mRNA. The increase in CLN3 mRNA half-life and abundance together with the increase in translational efficiency is fully sufficient to explain the small-cell phenotype of whi3 mutants. Under stress conditions, Whi3 becomes a component of P-bodies or stress granules, but Whi3 also acts under non-stress condition, when no P-bodies are visible. We suggest that Whi3 may be a very broadly-acting, but mild, modulator of mRNA stability. In CLN3, Whi3 may bind to the 3' GCAU motifs to attract the Ccr4-Not complex to promote RNA deadenylation and turnover, and Whi3 may bind to the 5' GCAU motifs to inhibit translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.