ArticlePloS one2013
Agrobacterium tumefaciens-mediated transformation of the lichen fungus, Umbilicaria muehlenbergii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 48 citations in OpenAlex.
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- Transcriptional analysis of the dimorphic fungus Umbilicaria muehlenbergii reveals the molecular mechanism of phenotypic transition.World journal of microbiology & biotechnology · 2023Article
- A comparative genomic analysis of lichen-forming fungi reveals new insights into fungal lifestyles.Scientific reports · 2022Article
- Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates inMolecules (Basel, Switzerland) · 2021Article
- Predicted Input of Uncultured Fungal Symbionts to a Lichen Symbiosis from Metagenome-Assembled Genomes.Genome biology and evolution · 2021Article
- Establishment ofJournal of fungi (Basel, Switzerland) · 2021Article
- Optimization ofMycobiology · 2021Article
- Coregulation of dimorphism and symbiosis by cyclic AMP signaling in the lichenized fungusProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Disruption of stcA blocks sterigmatocystin biosynthesis and improves echinocandin B production in Aspergillus delacroxii.World journal of microbiology & biotechnology · 2019Article
- Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice-The Plant cell · 2019Article
- A comprehensive catalogue of polyketide synthase gene clusters in lichenizing fungi.Journal of industrial microbiology & biotechnology · 2018Review
- Green Fluorescent Protein Expression in Pseudogymnoascus destructans to Study Its Abiotic and Biotic Lifestyles.Mycopathologia · 2018Article
- Suppressing a plant-parasitic nematode with fungivorous behavior by fungal transformation of a Bt cry gene.Microbial cell factories · 2018Article
- Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens.PloS one · 2018Article
- Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum.AMB Express · 2017Article
- Functional analysis of thioredoxin from the desert lichen-forming fungus, Endocarpon pusillum Hedwig, reveals its role in stress tolerance.Scientific reports · 2016Article
- Magnaporthe oryzae Effector AVR-Pii Helps to Establish Compatibility by Inhibition of the Rice NADP-Malic Enzyme Resulting in Disruption of Oxidative Burst and Host Innate Immunity.Molecules and cells · 2016Article
- Draft Genome Sequence of Umbilicaria muehlenbergii KoLRILF000956, a Lichen-Forming Fungus Amenable to Genetic Manipulation.Genome announcements · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transformation-mediated mutagenesis in both targeted and random manners has been widely applied to decipher gene function in diverse fungi. However, a transformation system has not yet been established for lichen fungi, severely limiting our ability to study their biology and mechanism underpinning symbiosis via gene manipulation. Here, we report the first successful transformation of the lichen fungus, Umbilicaria muehlenbergii, via the use of Agrobacterium tumefaciens. We generated a total of 918 transformants employing a binary vector that carries the hygromycin B phosphotransferase gene as a selection marker and the enhanced green fluorescent protein gene for labeling transformants. Randomly selected transformants appeared mitotically stable, based on their maintenance of hygromycin B resistance after five generations of growth without selection. Genomic Southern blot showed that 88% of 784 transformants contained a single T-DNA insert in their genome. A number of putative mutants affected in colony color, size, and/or morphology were found among these transformants, supporting the utility of Agrobacterium tumefaciens-mediated transformation (ATMT) for random insertional mutagenesis of U. muehlenbergii. This ATMT approach potentially offers a systematic gene functional study with genome sequences of U. muehlenbergii that is currently underway.
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