ArticleProceedings of the National Academy of Sciences of the United States of America2014
Three-dimensional aspects of matrix assembly by cells in the developing cornea.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 79 citations in OpenAlex.
- A collagen orientation switch reshapes fin architecture.iScience · 2026Article
- Biomedical Application of MSCs in Corneal Regeneration and Repair.International journal of molecular sciences · 2025Review
- Collagen fibril formation at the plasma membrane occurs independently from collagen secretion.Wellcome open research · 2025Article
- Reversed Corneal Fibroblasts Therapy Restores Transparency of Mouse Cornea after Injury.International journal of molecular sciences · 2024Article
- Article
- Designing collagens to shed light on the multi-scale structure-function mapping of matrix disorders.Matrix biology plus · 2024Review
- Studying the Proliferative Activity of Human Corneal Stromal Cell Populations.Bulletin of experimental biology and medicine · 2023Article
- Cell-Cell and Cell-Matrix Interactions at the Presumptive Stem Cell Niche of the Chick Corneal Limbus.Cells · 2023Article
- Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation.Disease models & mechanisms · 2023Article
- The impact of UV cross-linking on corneal stromal cell migration, differentiation and patterning.Experimental eye research · 2023Article
- Mechanochemistry of collagen.Acta biomaterialia · 2023Review
- Derivation of Human Corneal Keratocytes from ReLEx SMILE Lenticules for Cell Therapy and Tissue Engineering.International journal of molecular sciences · 2023Article
- Genipin increases extracellular matrix synthesis preventing corneal perforation.The ocular surface · 2023Article
- Developmental Changes in Patterns of Distribution of Fibronectin and Tenascin-C in the Chicken Cornea: Evidence for Distinct and Independent Functions during Corneal Development and Morphogenesis.International journal of molecular sciences · 2023Article
- Chondroitin Sulphate/Dermatan Sulphate Proteoglycans: Potential Regulators of Corneal Stem/Progenitor Cell Phenotype In Vitro.International journal of molecular sciences · 2023Article
- Biomechanics and mechanobiology of the bone matrix.Bone research · 2022Review
- Collagen fibril assembly: New approaches to unanswered questions.Matrix biology plus · 2021Article
- Article
- Engineering fiber anisotropy within natural collagen hydrogels.American journal of physiology. Cell physiology · 2021Review
- Tendon Extracellular Matrix Assembly, Maintenance and Dysregulation Throughout Life.Advances in experimental medicine and biology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Cell-directed deposition of aligned collagen fibrils during corneal embryogenesis is poorly understood, despite the fact that it is the basis for the formation of a corneal stroma that must be transparent to visible light and biomechanically stable. Previous studies of the structural development of the specialized matrix in the cornea have been restricted to examinations of tissue sections by conventional light or electron microscopy. Here, we use volume scanning electron microscopy, with sequential removal of ultrathin surface tissue sections achieved either by ablation with a focused ion beam or by serial block face diamond knife microtomy, to examine the microanatomy of the cornea in three dimensions and in large tissue volumes. The results show that corneal keratocytes occupy a significantly greater tissue volume than was previously thought, and there is a clear orthogonality in cell and matrix organization, quantifiable by Fourier analysis. Three-dimensional reconstructions reveal actin-associated tubular cell protrusions, reminiscent of filopodia, but extending more than 30 µm into the extracellular space. The highly extended network of these membrane-bound structures mirrors the alignment of collagen bundles and emergent lamellae and, we propose, plays a fundamental role in dictating the orientation of collagen in the developing cornea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.