ArticleNature protocols2014
Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe.Scientific reports · 2026Article
- Gamete fusion triggers cytosolic functions and P-body recruitment of the RNA-binding protein Mei2 to drive fission yeast zygotic development.Genes & development · 2026Article
- Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.Scientific reports · 2024Article
- Metabolic stress-induced long ncRNA transcription governs the formation of meiotic DNA breaks in the fission yeast fbp1 gene.PloS one · 2024Article
- Differential phosphorylation of Clr4EMBO reports · 2023Article
- SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Tandem affinity purification protocol for isolation of protein complexes fromSTAR protocols · 2022Article
- TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.Nature cell biology · 2021Article
- Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance.PLoS biology · 2021Article
- Tell the Difference Between Mitosis and Meiosis: Interplay Between Chromosomes, Cytoskeleton, and Cell Cycle Regulation.Frontiers in cell and developmental biology · 2021Review
- Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeastCell cycle (Georgetown, Tex.) · 2020Article
- Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance.The Journal of cell biology · 2019Article
- Review
- Physical basis for long-distance communication along meiotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mutations that prevent methylation of cohesin render sensitivity to DNA damage inJournal of cell science · 2018Article
- Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast.Cell cycle (Georgetown, Tex.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Synchronous cultures are often indispensable for studying meiosis. Here we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP analog 1-NM-PP1 in G1-arrested cells allows the induction of synchronous meiosis at optimal temperature (25°C). Importantly, this protocol eliminates detrimental effects of elevated temperature (34°C), which is required to inactivate the commonly used temperature-sensitive Pat1 kinase mutant (pat1-114). The addition of the mat-Pc gene to a mat1-M strain further improves chromosome segregation and spore viability. Thus, our protocol offers highly synchronous meiosis at optimal temperature, with most characteristics similar to those of wild-type meiosis. The synchronization protocol can be completed in 5 d (not including strain production, which may take as long as 2 or 3 months).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.