Evidence map›Paper›PMID 24385149›Full record

ArticleNature protocols2014

Extensible visualization and analysis for multidimensional images using Vaa3D.

Hanchuan Peng, Alessandro Bria, Zhi Zhou, Giulio Iannello, Fuhui Long

Abstract read
PubMed Publisher
In one paragraph

Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 167 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
167citing papers in PubMed, 1 pooled it
37.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

167 citing papers in PubMed, 1 synthesis or guideline pooled it, 347 citations in OpenAlex.

  1. Pooled it
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  10. Control of microglial dynamics by the Arp2/3 complex and the autism- and schizophrenia-associated protein CYFIP1.Proceedings of the National Academy of Sciences of the United States of America · 2026
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107 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Hanchuan Peng1] Allen Institute for Brain Sciences, Seattle, Washington, USA. [2] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.ORCID 0000000234783942
Alessandro Bria1] Integrated Research Centre, University Campus Bio-Medico of Rome, Rome, Italy. [2] Department of Electrical and Information Engineering, University of Cassino and Southern Lazio, Cassino, Italy.
Zhi ZhouAllen Institute for Brain Sciences, Seattle, Washington, USA.
Giulio IannelloIntegrated Research Centre, University Campus Bio-Medico of Rome, Rome, Italy.
Fuhui Long1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA. [2] BioImage, LLC, Bellevue, Washington, USA.
Janelia Research Campus · USAllen Institute for Brain Science · USUniversità Campus Bio-Medico · ITUniversità degli studi di Cassino e del Lazio Meridionale · IT

Funding

Howard Hughes Medical Institute
6 · The paper itself

Abstract

Open-Source 3D Visualization-Assisted Analysis (Vaa3D) is a software platform for the visualization and analysis of large-scale multidimensional images. In this protocol we describe how to use several popular features of Vaa3D, including (i) multidimensional image visualization, (ii) 3D image object generation and quantitative measurement, (iii) 3D image comparison, fusion and management, (iv) visualization of heterogeneous images and respective surface objects and (v) extension of Vaa3D functions using its plug-in interface. We also briefly demonstrate how to integrate these functions for complicated applications of microscopic image visualization and quantitative analysis using three exemplar pipelines, including an automated pipeline for image filtering, segmentation and surface generation; an automated pipeline for 3D image stitching; and an automated pipeline for neuron morphology reconstruction, quantification and comparison. Once a user is familiar with Vaa3D, visualization usually runs in real time and analysis takes less than a few minutes for a simple data set.

Indexed as

SoftwareAnimalsBrainComputer SimulationDrosophilaImaging, Three-DimensionalNeuronsUser-Computer Interface

Identifiers

PMID24385149
OpenAlexW1989396876

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.