ArticlePflugers Archiv : European journal of physiology2014
Heat and AITC activate green anole TRPA1 in a membrane-delimited manner.
Article in Pflugers Archiv : European journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- A single-point mutation in TRPA1 drives heat resilience in oviparous embryos.Science advances · 2026Article
- Cannabinoid and Cannabinoid-Related Receptors in the Myenteric Plexus of the Porcine Ileum.Animals : an open access journal from MDPI · 2021Article
- Allyl Isothiocyanate (AITC) Triggered Toxicity andFrontiers in microbiology · 2020Article
- Transient Receptor Potential Ankyrin Type-1 Channels as a Potential Target for the Treatment of Cardiovascular Diseases.Frontiers in physiology · 2020Review
- A Cross-Species Analysis Reveals a General Role for Piezo2 in Mechanosensory Specialization of Trigeminal Ganglia from Tactile Specialist Birds.Cell reports · 2019Article
- Neural basis of trigeminal chemo- and thermonociception in brown treesnakes, Boiga irregularis (Squamata: Colubridae).Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2018Article
- TRPs et al.: a molecular toolkit for thermosensory adaptations.Pflugers Archiv : European journal of physiology · 2018Review
- Dependence of heat-evoked TRPA1 activation on extracellular CaChannels (Austin, Tex.) · 2017Article
- Requirement of extracellular CaThe Journal of physiology · 2017Article
- Evolutionary tuning of TRPA1 and TRPV1 thermal and chemical sensitivity in vertebrates.Temperature (Austin, Tex.) · 2017Review
- Propofol-induced pain sensation involves multiple mechanisms in sensory neurons.Pflugers Archiv : European journal of physiology · 2015Article
- Molecular mechanisms of temperature adaptation.The Journal of physiology · 2015Review
- Molecular basis determining inhibition/activation of nociceptive receptor TRPA1 protein: a single amino acid dictates species-specific actions of the most potent mammalian TRPA1 antagonist.The Journal of biological chemistry · 2014Article
- Species-specific temperature sensitivity of TRPA1.Temperature (Austin, Tex.)Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient receptor potential ankyrin 1 (TRPA1) is a member of the large TRP super family of ion channels and functions as a Ca(2+)-permeable nonselective cation channel that is activated by various noxious stimuli. TRPA1 was initially identified as a potential mediator of noxious cold stimuli in mammalian nociceptive sensory neurons, while TRPA1s from nonmammalian vertebrates (snakes, green anole lizards, and frogs) were recently reported to be activated by heat, but not cold stimulus. In this study, we examined detailed properties of the green anole TRPA1 channel (gaTRPA1) related to thermal and chemical stimulation in whole-cell and single-channel recordings. Heat activates gaTRPA1 with a temperature threshold for activation of 35.8 °C, while heat together with allyl isothiocyanate (AITC), a chemical agonist, had synergistic effects on gaTRPA1 channel activation in that either the temperature threshold or activating AITC concentration was reduced in the presence of the other stimulus. Significant heat-evoked gaTRPA1 activation was observed in the presence but not absence of extracellular Ca(2+). gaTRPA1 channels were also activated by heat and AITC in excised membrane patches with an inside-out configuration. By comparing the kinetics of heat- and AITC-evoked single-channel currents, we defined similarities and differences of gaTRPA1 channel responses to heat and AITC. We observed similar current-voltage relationship and unitary amplitudes for heat- and AITC-evoked currents and found that heat-activated currents showed shorter durations of both open and closed times. Our results suggest that the gaTRPA1 channel is directly activated by heat and chemical stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.