ArticleDNA and cell biology2014
Let-7a functions as a tumor suppressor in Ewing's sarcoma cell lines partly by targeting cyclin-dependent kinase 6.
Article in DNA and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Development of 5-FU-modified tumor suppressor microRNAs as a platform for novel microRNA-based cancer therapeutics.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Let-7a suppresses Ewing sarcoma CSCs' malignant phenotype via forming a positive feedback circuit with STAT3 and lin28.Journal of bone oncology · 2021Article
- LncRNA FOXP4-AS1 Promotes Progression of Ewing Sarcoma and Is Associated With Immune Infiltrates.Frontiers in oncology · 2021Article
- Modeling the Tumor Microenvironment and Pathogenic Signaling in Bone Sarcoma.Tissue engineering. Part B, Reviews · 2020Review
- Slow skeletal muscle troponin T, titin and myosin light chain 3 are candidate prognostic biomarkers for Ewing's sarcoma.Oncology letters · 2019Article
- Toosendanin induces the apoptosis of human Ewing's sarcoma cells via the mitochondrial apoptotic pathway.Molecular medicine reports · 2019Article
- Circulating Exosomal miRNA Profile During Term and Preterm Birth Pregnancies: A Longitudinal Study.Endocrinology · 2019Article
- Diagnostic and prognostic values of blood microRNA-Let7A for osteosarcoma.Journal of bone oncology · 2018Article
- Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm.PloS one · 2018Article
- miR-124 represses the mesenchymal features and suppresses metastasis in Ewing sarcoma.Oncotarget · 2017Article
- Review
- Expression of CCL21 in Ewing sarcoma shows an inverse correlation with metastases and is a candidate target for immunotherapy.Cancer immunology, immunotherapy : CII · 2016Article
- Ewing's Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue.International journal of molecular sciences · 2016Article
- Expression of cdk6 in head and neck squamous cell carcinoma.Clinical oral investigations · 2016Article
- Article
- Overexpression of miR‑199b‑5p inhibits Ewing's sarcoma cell lines by targeting CCNL1.Molecular medicine reports · 2015Article
- MicroRNAs in the pathobiology of sarcomas.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- What Do We Know about the Role of miRNAs in Pediatric Sarcoma?International journal of molecular sciences · 2015Review
- miR-211 suppresses epithelial ovarian cancer proliferation and cell-cycle progression by targeting Cyclin D1 and CDK6.Molecular cancer · 2015Article
- MicroRNA expression and its clinical implications in Ewing's sarcoma.Cell proliferation · 2015Review
Corrections and comments
- Retraction · 2022-03-30Author Unresponsive · Duplication of/in Image · Investigation by Journal/Publisher ·
- Retracted
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs play an important role in the development and progression of Ewing's sarcoma (ES). Especially, the expression of let-7a has been reported to be significantly downregulated in various cancers, and can affect the initiation and maintenance of tumor progression. However, the relative effects of let-7a on ES cells and relative mechanisms are largely unknown. In this study, we identified the underexpression of let-7a in human ES cells comparing with the human mesenchymal stem cells. Then, we sought to compensate for its loss through exogenous transfection with let-7a mimic into ES cell lines A673 and SK-ES-1. Restored let-7a expression inhibited cell proliferation, migration, as well as invasion; arrested cell cycle progression; and induced cell apoptosis of both cell lines. Moreover, bioinformatic prediction suggested that cyclin-dependent kinase 6 (CDK6), which is overexpressed and functions as an oncoprotein in ES cells, is a putative target gene of let-7a. Using mRNA and protein expression analysis and luciferase assays, we further identified the target role of CDK6. Finally, we found that restored CDK6 expression in ES cells that had been treated with let-7a mimic before could partly dampen let-7a-mediated tumor suppression. Taken together, our results showed that let-7a acted as a tumor suppressor in ES by targeting CDK6, and it may provide novel diagnostic and therapeutic options for human Ewing sarcoma clinical operation in future.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.