ArticleBMC microbiology2014
Characterization and resuscitation of 'non-culturable' cells of Legionella pneumophila.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 98 citations in OpenAlex.
- A photoactivation-free viability PCR reagent for rapid, culture-independent detection of live bacteria.Microbiology spectrum · 2026Article
- Role of Next-Generation Sequencing in Excluding the Nosocomial Origin of a Case of Legionnaires' Disease Integrating Environmental Surveillance and Clinical Diagnosis.Microorganisms · 2026Article
- Moving Beyond the Silos of Opportunistic Pathogen and Disinfection Byproduct Research to Improve Drinking Water System Management.Environmental science & technology · 2025Review
- Intracellular ATP concentration is a key regulator of bacterial cell fate.Journal of bacteriology · 2024Article
- Molecular mechanism and application of emerging technologies in study of bacterial persisters.BMC microbiology · 2024Review
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Multiple factors trigger the formation and resuscitation of the VBNC state in alcohol-producingApplied and environmental microbiology · 2024Article
- Bacterial persisters: molecular mechanisms and therapeutic development.Signal transduction and targeted therapy · 2024Review
- Effects of Copper onPathogens (Basel, Switzerland) · 2024Article
- Exploration of genes associated with induction of the viable but non-culturable state of Campylobacter jejuni.Archives of microbiology · 2024Article
- Correlation between bacterial microbiome andMicrobiology spectrum · 2024Article
- Kicking sleepers out of bed: Macrophages promote reactivation of dormant Cryptococcus neoformans by extracellular vesicle release and non-lytic exocytosis.PLoS pathogens · 2023Article
- Insight into the Mechanism of Interactions between the LL-37 Peptide and Model Membranes ofInternational journal of molecular sciences · 2023Article
- Inaccuracies of the ISO 11731 Method for Environmental Validation ofMicroorganisms · 2022Article
- Absolute Quantification of Viable but Nonculturable Vibrio cholerae Using Droplet Digital PCR with Oil-Enveloped Bacterial Cells.Microbiology spectrum · 2022Article
- Article
- Detection of Legionella species, the influence of precipitation on the amount of Legionella DNA, and bacterial microbiome in aerosols from outdoor sites near asphalt roads in Toyama Prefecture, Japan.BMC microbiology · 2021Article
- Comparison of two culture methods for the enumeration of Legionella pneumophila from potable water samples.Journal of water and health · 2021Article
- Occurrence ofPathogens (Basel, Switzerland) · 2021Article
- Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms.Marine life science & technology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLegionella pneumophila is a waterborne pathogen responsible for Legionnaires' disease, an infection which can lead to potentially fatal pneumonia. After disinfection, L. pneumophila has been detected, like many other bacteria, in a "viable but non culturable" state (VBNC). The physiological significance of the VBNC state is unclear and controversial: it could be an adaptive response favoring long-term survival; or the consequence of cellular deterioration which, despite maintenance of certain features of viable cells, leads to death; or an injured state leading to an artificial loss of culturability during the plating procedure. VBNC cells have been found to be resuscitated by contact with amoebae.
resultsWe used quantitative microscopic analysis, to investigate this "resuscitation" phenomenon in L. pneumophila in a model involving amending solid plating media with ROS scavengers (pyruvate or glutamate), and co-culture with amoebae. Our results suggest that the restoration observed in the presence of pyruvate and glutamate may be mostly due to the capacity of these molecules to help the injured cells to recover after a stress. We report evidence that this extracellular signal leads to a transition from a not-culturable form to a culturable form of L. pneumophila, providing a technique for recovering virulent and previously uncultivated forms of L. pneumophila.
conclusionThese new media could be used to reduce the risk of underestimation of counts of virulent of L. pneumophila cells in environmental samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.