ArticleMolecular & cellular proteomics : MCP2014
A cell surface biotinylation assay to reveal membrane-associated neuronal cues: Negr1 regulates dendritic arborization.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Structural basis of IgLON5 autoantibody recognition in autoimmune encephalitis.Nature communications · 2026Article
- Altered trafficking of Kv1-Kvβ2 leads to neuronal hyperexcitability and memory deficits in amyloid-β pathology.Molecular neurodegeneration · 2026Article
- Negr1 Deficiency Modulates Sex-Specific Neurobehavioral Adaptations to Social Isolation.Brain sciences · 2025Article
- Elevated NEGR1 in brain induces anxiety or depression-like phenotypes and synaptic dysfunction.Molecular psychiatry · 2025Article
- The pathogenic APP N-terminal Val225Ala mutation alters tau protein liquid-liquid phase separation and exacerbates synaptic damage.Molecular psychiatry · 2025Article
- Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy.Nature communications · 2025Article
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- Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.PLoS biology · 2024Article
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- Defining the Cell Surface Cysteinome using Two-step Enrichment Proteomics.bioRxiv : the preprint server for biology · 2023Article
- Review
- Negr1-Derived Peptides Trigger ALK Degradation and Halt Neuroblastoma Progression In Vitro and In Vivo.Pharmaceutics · 2023Article
- A strategy can be used to analyze intracellular interaction proteomics of cell-surface receptors.Amino acids · 2023Article
- Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons.Nature communications · 2023Article
- Depression-AssociatedBrain sciences · 2022Article
- Article
- Delineating the Genetic Component of Gene Expression in Major Depression.Biological psychiatry · 2021Article
- Pharmacological inactivation of the prion protein by targeting a folding intermediate.Communications biology · 2021Article
- Highly Conserved Molecular Features in IgLONs Contrast Their Distinct Structural and Biological Outcomes.Journal of molecular biology · 2020Article
- Depression-Associated GeneCells · 2020Article
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11 authors.
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Abstract
A complex and still not comprehensively resolved panel of transmembrane proteins regulates the outgrowth and the subsequent morphological and functional development of neuronal processes. In order to gain a more detailed description of these events at the molecular level, we have developed a cell surface biotinylation assay to isolate, detect, and quantify neuronal membrane proteins. When we applied our assay to investigate neuron maturation in vitro, we identified 439 differentially expressed proteins, including 20 members of the immunoglobulin superfamily. Among these candidates, we focused on Negr1, a poorly described cell adhesion molecule. We demonstrated that Negr1 controls the development of neurite arborization in vitro and in vivo. Given the tight correlation existing among synaptic cell adhesion molecules, neuron maturation, and a number of neurological disorders, our assay results are a useful tool that can be used to support the understanding of the molecular bases of physiological and pathological brain function.
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