Evidence map›Paper›PMID 24381568›Full record

ArticleFrontiers in microbiology2013

Transgenic expression of the human LEDGF/p75 gene relieves the species barrier against HIV-1 infection in mouse cells.

Takuya Tada, Motohiko Kadoki, Yang Liu, Kenzo Tokunaga, Yoichiro Iwakura

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Animal model studies on viral infections.Frontiers in microbiology · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Takuya TadaCenter for Experimental Medicine and System Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan ; Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo Tokyo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Saitama, Japan ; Department of Pathology, National Institute of Infectious Diseases Tokyo, Japan.
Motohiko KadokiCenter for Experimental Medicine and System Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan ; Research Institute for Biomedical Sciences, Tokyo University of Science Chiba, Japan.
Yang LiuCenter for Experimental Medicine and System Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan ; Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine Renji Hospital Shanghai, China.
Kenzo TokunagaDepartment of Pathology, National Institute of Infectious Diseases Tokyo, Japan.
Yoichiro IwakuraCenter for Experimental Medicine and System Biology, Institute of Medical Science, University of Tokyo Tokyo, Japan ; Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo Tokyo, Japan ; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency Saitama, Japan ; Research Institute for Biomedical Sciences, Tokyo University of Science Chiba, Japan.
Tokyo University of Science · JPNational Institute of Infectious Diseases · JPRenji Hospital · CNThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Attempts to create mouse models for AIDS have been hampered by species barriers in HIV-1 infection. We previously showed that the nuclear accumulation of HIV-1 preintegration complex (PIC) was suppressed in mouse cells. Lens epithelium-derived growth factor (LEDGF/p75) is a host factor identified as a binding partner of integrase (IN), and has been suggested to be involved in promoting viral integration by tethering PIC to the chromatin, which are observed as nuclear accumulation of IN by LEDGF/p75. Therefore, we here hypothesized that this host factor might act as one of the species-specific barriers in mouse cells. We generated transgenic (Tg) mice that constitutively express human (h) LEDGF/p75. The GFP-fused IN was efficiently accumulated into the nucleus of hLEDGF/p75 expressing Tg mouse embryonic fibroblast (MEF) cells in contrast to the control MEF cells. Importantly, hLEDGF/p75 Tg MEF cells were significantly more susceptible to HIV-1 infection. These results suggest that LEDGF/p75 is one of the host factors that constitute species barrier against HIV-1 in mouse cells.

Indexed as

HIV-1INLEDGF/p75species barriertransgenic mouse

Identifiers

PMID24381568
PMCPMC3865800
OpenAlexW2007964382

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.