ArticleProceedings of the National Academy of Sciences of the United States of America2014
Analysis and modeling of mitotic spindle orientations in three dimensions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.Current biology : CB · 2024Article
- An actomyosin network organizes niche morphology and responds to feedback from recruited stem cells.bioRxiv : the preprint server for biology · 2024Article
- Impairment of α-tubulin and F-actin interactions of GJB3 induces aneuploidy in urothelial cells and promotes bladder cancer cell invasion.Cellular & molecular biology letters · 2024Article
- A two-kinesin mechanism controls neurogenesis in the developing brain.Communications biology · 2023Article
- Deep learning techniques and mathematical modeling allow 3D analysis of mitotic spindle dynamics.The Journal of cell biology · 2023Article
- Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CentTracker: a trainable, machine-learning-based tool for large-scale analyses ofMolecular biology of the cell · 2021Article
- Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini.Developmental cell · 2020Article
- Endfoot regeneration restricts radial glial state and prevents translocation into the outer subventricular zone in early mammalian brain development.Nature cell biology · 2020Article
- Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue ofBio-protocol · 2019Article
- Cell-cell adhesion accounts for the different orientation of columnar and hepatocytic cell divisions.The Journal of cell biology · 2017Article
- Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia.Neural development · 2017Article
- Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.Molecular biology of the cell · 2017Article
- Article
- Transit-Amplifying Cells in the Fast Lane from Stem Cells towards Differentiation.Stem cells international · 2017Review
- The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.Scientific reports · 2016Article
- Article
- Mutations in genes encoding condensin complex proteins cause microcephaly through decatenation failure at mitosis.Genes & development · 2016Article
- Novel insights into mammalian embryonic neural stem cell division: focus on microtubules.Molecular biology of the cell · 2015Article
- Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum.Molecular biology of the cell · 2015Article
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4 authors.
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Abstract
The orientation of the mitotic spindle determines the relative size and position of the daughter cells and influences the asymmetric inheritance of localized cell fate determinants. The onset of mammalian neurogenesis, for example, coincides with changes in spindle orientation. To address the functional implications of this and related phenomena, precise methods for determining the orientation of the mitotic spindle in complex tissues are needed. Here, we present methodology for the analysis of spindle orientation in 3D. Our method allows statistical analysis and modeling of spindle orientation and involves two parameters for horizontal and vertical bias that can unambiguously describe the distribution of spindle orientations in an experimental sample. We find that 3D analysis leads to systematically different results from 2D analysis and, surprisingly, truly random spindle orientations do not result in equal numbers of horizontal and vertical orientations. We show that our method can describe the distribution of spindle orientation angles under different biological conditions. As an example of biological application we demonstrate that the adapter protein Inscuteable (mInsc) can actively promote vertical spindle orientation in apical progenitors during mouse neurogenesis.
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