ArticleCell research2014
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells.
Article in Cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- TET3 protects theiScience · 2025Article
- Identification of Hub of the Hub-Genes From Different Individual Studies for Early Diagnosis, Prognosis, and Therapies of Breast Cancer.Bioinformatics and biology insights · 2024Article
- Promoting GSDME expression through DNA demethylation to increase chemosensitivity of breast cancer MCF-7 / Taxol cells.PloS one · 2023Article
- BrewerIX enables allelic expression analysis of imprinted and X-linked genes from bulk and single-cell transcriptomes.Communications biology · 2022Article
- Minor Splicing FactorsInternational journal of molecular sciences · 2020Article
- Role of Long Non-coding RNAs in Reprogramming to Induced Pluripotency.Genomics, proteomics & bioinformatics · 2020Review
- Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs.Cell stem cell · 2019Article
- Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.Cell stem cell · 2019Article
- Conserved regulation of RNA processing in somatic cell reprogramming.BMC genomics · 2019Article
- Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells.Scientific reports · 2018Article
- Culture of haploid blastocysts in FGF4 favors the derivation of epiblast stem cells with a primed epigenetic and transcriptional landscape.Scientific reports · 2018Article
- X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs.Stem cell reports · 2018Article
- Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo.Cell stem cell · 2018Article
- Age-Related Epigenetic Derangement upon Reprogramming and Differentiation of Cells from the Elderly.Genes · 2018Review
- OCT4 supports extended LIF-independent self-renewal and maintenance of transcriptional and epigenetic networks in embryonic stem cells.Scientific reports · 2017Article
- miR-6539 is a novel mediator of somatic cell reprogramming that represses the translation of Dnmt3b.The Journal of reproduction and development · 2017Article
- High throughput sequencing identifies an imprinted gene, Grb10, associated with the pluripotency state in nuclear transfer embryonic stem cells.Oncotarget · 2017Article
- Synergetic effects of DNA methylation and histone modification during mouse induced pluripotent stem cell generation.Scientific reports · 2017Article
- Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs.Stem cell research & therapy · 2017Article
Corrections and comments
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Authors and funding
20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It remains controversial whether the abnormal epigenetic modifications accumulated in the induced pluripotent stem cells (iPSCs) can ultimately affect iPSC pluripotency. To probe this question, iPSC lines with the same genetic background and proviral integration sites were established, and the pluripotency state of each iPSC line was characterized using tetraploid (4N) complementation assay. Subsequently, gene expression and global epigenetic modifications of "4N-ON" and the corresponding "4N-OFF" iPSC lines were compared through deep sequencing analyses of mRNA expression, small RNA profile, histone modifications (H3K27me3, H3K4me3, and H3K4me2), and DNA methylation. We found that methylation of an imprinted gene, Zrsr1, was consistently disrupted in the iPSC lines with reduced pluripotency. Furthermore, the disrupted methylation could not be rescued by improving culture conditions or subcloning of iPSCs. Moreover, the relationship between hypomethylation of Zrsr1 and pluripotency state of iPSCs was further validated in independent iPSC lines derived from other reprogramming systems.
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