ArticleParasites & vectors2013
Multispacer typing of Rickettsia isolates from humans and ticks in Tunisia revealing new genotypes.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
- Molecular identification, genotyping and phylogenetic analysis ofFrontiers in microbiology · 2025Article
- First Evidence ofVeterinary sciences · 2024Article
- Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges.One health (Amsterdam, Netherlands) · 2023Review
- Diversity and Phylogeny of Cattle Ixodid Ticks and Associated Spotted Fever GroupPathogens (Basel, Switzerland) · 2023Article
- Distribution and Prevalence ofMicroorganisms · 2023Review
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- ZoonoticFrontiers in veterinary science · 2021Article
- Tick Fauna and AssociatedMicroorganisms · 2020Article
- Isolation and characterization of a Rickettsia from the ovary of a Western black-legged tick, Ixodes pacificus.Ticks and tick-borne diseases · 2019Article
- Ticks and Tick-Borne Infections: Complex Ecology, Agents, and Host Interactions.Veterinary sciences · 2018Review
- Detection of Bartonella tamiae, Coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern Algeria.Parasites & vectors · 2016Article
- Restriction of Francisella novicida genetic diversity during infection of the vector midgut.PLoS pathogens · 2014Article
- Detection of Rickettsia rickettsii, Rickettsia parkeri, and Rickettsia akari in skin biopsy specimens using a multiplex real-time polymerase chain reaction assay.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRickettsioses are important remerging vector born infections. In Tunisia, many species have been described in humans and vectors. Genotyping is important for tracking pathogen movement between hosts and vectors. In this study, we characterized Rickettsia species detected in patients and vectors using multispacer typing (MST), proposed by Founier et al. and based on three intergenic spacers (dksA-xerC, rmpE- tRNA(fMet), mppA-pruC) sequencing.
methodsOur study included 25 patients hospitalized during 2009. Ticks and fleas were collected in the vicinity of confirmed cases. Serology was performed on serum samples by microimmunofluorescence using Rickettsia conorii and Rickettsia typhi antigens. To detect and identify Rickettsia species, PCR targeting ompA, ompB and gltA genes followed by sequencing was performed on 18 obtained skin biopsies and on all collected vectors. Rickettsia positive samples were further characterized using primers targeting three intergenic spacers (dksA-xerC, rmpE- tRNA(fMet) and mppA-purC).
resultsA rickettsial infection was confirmed in 15 cases (60%). Serology was positive in 13 cases (52%). PCR detected Rickettsia DNA in four biopsies (16%) allowing the identification of R. conorii subsp israelensis in three cases and R. conorii subsp conorii in one case. Among 380 collected ticks, nine presented positive PCR (2.4%) allowing the identification of six R. conorii subsp israelensis, two R. massiliae and one R. conorii subsp conorii. Among 322 collected fleas, only one was positive for R. felis. R. conorii subsp israelensis strains detected in humans and vectors clustered together and showed a new MST genotype. Similarly, R. conorii subsp conorii strains detected in a skin biopsy and a tick were genetically related and presented a new MST genotype.
conclusionsNew Rickettsia spotted fever strain genotypes were found in Tunisia. Isolates detected in humans and vectors were genetically homogenous despite location differences in their original isolation suggesting epidemiologic circulation of these strains.
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