ArticleMolecular and cellular biology2014
Cross talk between CD3 and CD28 is spatially modulated by protein lateral mobility.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Cell polarity: cell type-specific regulators, common pathways, and polarized vesicle transport.Leukemia · 2025Review
- Biomimetic cell stimulation with a graphene oxide antigen-presenting platform for developing T cell-based therapies.Nature nanotechnology · 2024Article
- PRKAG2.2 is essential for FoxA1Science advances · 2023Article
- NF-κB dynamics in the language of immune cells.Trends in immunology · 2023Review
- Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity.Nature communications · 2022Article
- Programmable DNA-augmented hydrogels for controlled activation of human lymphocytes.Nanomedicine : nanotechnology, biology, and medicine · 2021Article
- Biphasic response of T cell activation to substrate stiffness.Biomaterials · 2021Article
- Multi-Factor Clustering Incorporating Cell Motility Predicts T Cell Expansion Potential.Frontiers in cell and developmental biology · 2021Article
- Biomaterials to enhance antigen-specific T cell expansion for cancer immunotherapy.Biomaterials · 2021Review
- Review
- Engineering Cytoplasmic Signaling of CD28ζ CARs for Improved Therapeutic Functions.Frontiers in immunology · 2020Review
- Single-molecule investigations of T-cell activation.Current opinion in biomedical engineering · 2019Article
- T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanosensing through immunoreceptors.Nature immunology · 2019Article
- Depletion of the cellular cholesterol content reduces the dynamics of desmosomal cadherins and interferes with desmosomal strength.Histochemistry and cell biology · 2019Article
- Separating T Cell Targeting Components onto Magnetically Clustered Nanoparticles Boosts Activation.Nano letters · 2018Article
- Enhanced activation and expansion of T cells using mechanically soft elastomer fibers.Advanced biosystems · 2018Article
- Engineering Platforms for T Cell Modulation.International review of cell and molecular biology · 2018Review
- Receptor-mediated cell mechanosensing.Molecular biology of the cell · 2017Review
- Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse.Biophysical journal · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Functional convergence of CD28 costimulation and TCR signaling is critical to T-cell activation and adaptive immunity. These receptors form complex microscale patterns within the immune synapse, although the impact of this spatial organization on cell signaling remains unclear. We investigate this cross talk using micropatterned surfaces that present ligands to these membrane proteins in order to control the organization of signaling molecules within the cell-substrate interface. While primary human CD4(+) T cells were activated by features containing ligands to both CD3 and CD28, this functional convergence was curtailed on surfaces in which engagement of these two systems was separated by micrometer-scale distances. Moreover, phosphorylated Lck was concentrated to regions of CD3 engagement and exhibited a low diffusion rate, suggesting that costimulation is controlled by a balance between the transport of active Lck to CD28 and its deactivation. In support of this model, disruption of the actin cytoskeleton increased Lck mobility and allowed functional T-cell costimulation by spatially separated CD3 and CD28. In primary mouse CD4(+) T cells, a complementary system, reducing the membrane mobility increased the sensitivity to CD3-CD28 separation. These results demonstrate a subcellular reaction-diffusion system that allows cells to sense the microscale organization of the extracellular environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.