ArticleJournal of visualized experiments : JoVE2013
Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Bipolar cell networks underlying steady-state intensity encoding in intrinsically photosensitive retinal ganglion cells.iScience · 2026Article
- A Novel Retinal Nerve Fiber Layer Biomarker of Amyotrophic Lateral Sclerosis (ALS) Identified Using Longitudinal in vivo Ocular Imaging.Eye and brain · 2025Article
- Oral Metformin Inhibits Choroidal Neovascularization by Modulating the Gut-Retina Axis.Investigative ophthalmology & visual science · 2023Article
- Long-Term Effects on Retinal Structure and Function in a Mouse Endothelin-1 Model of Retinal Ganglion Cell Degeneration.Investigative ophthalmology & visual science · 2023Article
- Impaired Light Adaptation of ON-Sustained Ganglion Cells in Early Diabetes Is Attributable to Diminished Response to Dopamine D4 Receptor Activation.Investigative ophthalmology & visual science · 2022Article
- Dopamine D1 and D4 receptors contribute to light adaptation in ON-sustained retinal ganglion cells.Journal of neurophysiology · 2021Article
- Overexpression of Msx1 in Mouse Lung Leads to Loss of Pulmonary Vessels Following Vascular Hypoxic Injury.Cells · 2021Article
- Gnotobiotic Operations and Assembly for Development of Germ-Free Animal Model of Laser-Induced Choroidal Neovascularization.Translational vision science & technology · 2021Article
- Retina-specific targeting of pericytes reveals structural diversity and enables control of capillary blood flow.The Journal of comparative neurology · 2021Article
- Inhalable Thioflavin S for the Detection of Amyloid Beta Deposits in the Retina.Molecules (Basel, Switzerland) · 2021Article
- Spatial Expression Pattern of the Major CaCells · 2020Article
- Domain-specific distribution of gap junctions defines cellular coupling to establish a vascular relay in the retina.The Journal of comparative neurology · 2019Article
- Article
- Cone synapses in mammalian retinal rod bipolar cells.The Journal of comparative neurology · 2018Article
- Inhomogeneous Encoding of the Visual Field in the Mouse Retina.Current biology : CB · 2018Article
- Intravitreal Stanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization.Investigative ophthalmology & visual science · 2018Article
- Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4.Journal of visualized experiments : JoVE · 2017Article
- Article
- Aberrant activity in retinal degeneration impairs central visual processing and relies on Cx36-containing gap junctions.Experimental eye research · 2016Article
- Longitudinal live imaging of retinal α-synuclein::GFP deposits in a transgenic mouse model of Parkinson's Disease/Dementia with Lewy Bodies.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Working with delicate tissue can be a complicating factor when performing immunohistochemical assessment. Here, we present a method that utilizes a ring-supported hydrophilized PTFE membrane to provide structural support to both living and fixed tissue during immunohistochemical processing, which allows for the use of a variety of protocols that would otherwise cause damage to the tissue. First, this is demonstrated with bolus loading of fluorescent markers into living retinal tissue. This method allows for quick visualization of targeted structures, while the membrane support maintains tissue integrity during the injection and allows for easy transfer of the preparation for further imaging or processing. Second, a procedure for antibody staining in tissue fixed with carbodiimide is described. Though paraformaldehyde fixation is more common, carbodiimide fixation provides a superior substrate for the visualization of synaptic proteins. A limitation of carbodiimide is that the resulting fixed tissue is relatively fragile; however, this is overcome with the use of the supporting membrane. Retinal tissue is used to demonstrate these techniques, but they may be applied to any fragile tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.