ArticleAnalytical biochemistry2014
Uninduced high-yield bacterial expression of fluorescent proteins.
Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- The mouse neonatal small intestine is regionally specialized for protein absorption and transepithelial transport.Development (Cambridge, England) · 2025Article
- Enhancing cap-independent translation of linear mRNA.Nature communications · 2025Article
- Hypervesiculation Meets Sec-Targeting: Enhancing Heterologous Protein Loading inInternational journal of molecular sciences · 2025Article
- Mechanical disruption of E-cadherin complexes with epidermal growth factor receptor actuates growth factor-dependent signaling.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Quantitative characterization of tetraspanin 8 homointeractions in the plasma membrane.The Biochemical journal · 2021Article
- P120 catenin potentiates constitutive E-cadherin dimerization at the plasma membrane and regulates trans binding.Current biology : CB · 2021Article
- A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain.The Journal of biological chemistry · 2021Article
- Enabling high-throughput biology with flexible open-source automation.Molecular systems biology · 2021Article
- Portable CRISPR-Cas9Applied and environmental microbiology · 2021Article
- The Biased Ligands NGF and NT-3 Differentially Stabilize Trk-A Dimers.Biophysical journal · 2021Article
- The biophysical basis of receptor tyrosine kinase ligand functional selectivity: Trk-B case study.The Biochemical journal · 2020Article
- Quantifying the strength of heterointeractions among receptor tyrosine kinases from different subfamilies: Implications for cell signaling.The Journal of biological chemistry · 2020Article
- Bacterial Glycogen Provides Short-Term Benefits in Changing Environments.Applied and environmental microbiology · 2020Article
- Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut.Developmental cell · 2019Article
- Dimerization of the Trk receptors in the plasma membrane: effects of their cognate ligands.The Biochemical journal · 2018Article
- Interactions between NRP1 and VEGFR2 molecules in the plasma membrane.Biochimica et biophysica acta. Biomembranes · 2018Article
- Cooperative interactions between VEGFR2 extracellular Ig-like subdomains ensure VEGFR2 dimerization.Biochimica et biophysica acta. General subjects · 2017Article
- A New Method to Study Heterodimerization of Membrane Proteins and Its Application to Fibroblast Growth Factor Receptors.The Journal of biological chemistry · 2017Article
- Effect of the achondroplasia mutation on FGFR3 dimerization and FGFR3 structural response to fgf1 and fgf2: A quantitative FRET study in osmotically derived plasma membrane vesicles.Biochimica et biophysica acta · 2016Article
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Authors and funding
3 authors.
Funding
Abstract
Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization and does not use isopropyl β-d-1-thiogalactopyranoside (IPTG) induction. The method relies on uninduced expression in the BL21-Gold (DE3) strain of Escherichia coli and yields large amounts (up to 0.4 μmol) of fluorescent protein from a 250-ml culture. This method is much simpler than published methods and can be used to produce any fluorescent protein that is needed in biomedical research.
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Registered trials
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