ArticleJournal of visualized experiments : JoVE2013
Visualizing cytoplasmic flow during single-cell wound healing in Stentor coeruleus.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- A role for theMolecular biology of the cell · 2026Article
- Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus.Current biology : CB · 2026Article
- A role for thebioRxiv : the preprint server for biology · 2025Article
- Characterization of cellular wound resistance in the giant ciliatebioRxiv : the preprint server for biology · 2025Article
- Physical Forces in Regeneration of Cells and Tissues.Cold Spring Harbor perspectives in biology · 2025Review
- Article
- Microfluidic guillotine reveals multiple timescales and mechanical modes of wound response in Stentor coeruleus.BMC biology · 2021Article
- Bioactive Proteins in Channa striata Promote Wound Healing through Angiogenesis and Cell Proliferation.Protein and peptide letters · 2020Article
- Methods for the Study of Regeneration in Stentor.Journal of visualized experiments : JoVE · 2018Article
- Microfluidic guillotine for single-cell wound repair studies.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Self-repairing cells: How single cells heal membrane ruptures and restore lost structures.Science (New York, N.Y.) · 2017Review
- How nontraditional model systems can save us.Molecular biology of the cell · 2015Article
- Stentor coeruleus.Current biology : CB · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
Although wound-healing is often addressed at the level of whole tissues, in many cases individual cells are able to heal wounds within themselves, repairing broken cell membrane before the cellular contents leak out. The giant unicellular organism Stentor coeruleus, in which cells can be more than one millimeter in size, have been a classical model organism for studying wound healing in single cells. Stentor cells can be cut in half without loss of viability, and can even be cut and grafted together. But this high tolerance to cutting raises the question of why the cytoplasm does not simply flow out from the size of the cut. Here we present a method for cutting Stentor cells while simultaneously imaging the movement of cytoplasm in the vicinity of the cut at high spatial and temporal resolution. The key to our method is to use a "double decker" microscope configuration in which the surgery is performed under a dissecting microscope focused on a chamber that is simultaneously viewed from below at high resolution using an inverted microscope with a high NA lens. This setup allows a high level of control over the surgical procedure while still permitting high resolution tracking of cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.