Evidence map›Paper›PMID 24378434›Full record

ArticleJournal of orthopaedic trauma2014

Modulation of macrophage activity during fracture repair has differential effects in young adult and elderly mice.

Jesse Alan Slade Shantz, Yan-Yiu Yu, Wells Andres, Theodore Miclau, Ralph Marcucio

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic trauma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 55 citations in OpenAlex.

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  7. Research Progress of Macrophages in Bone Regeneration.Journal of tissue engineering and regenerative medicine · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jesse Alan Slade ShantzDepartment of Orthopaedic Surgery, Orthopaedic Trauma Institute, University of California, San Francisco, CA.
Yan-Yiu Yu
Wells Andres
Theodore Miclau
Ralph Marcucio
University of California, San Francisco · US

Funding

Functional roles of muscle in bone repairR01AR057344 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MICLAU, THEODORE · 2010 to 2014
$1.7M
Biology of stable and non-stable fracture repairR01AR053645 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MICLAU, THEODORE · 2006 to 2010
$1.6M
NIAMS NIH HHS R01 AR053645NIAMS NIH HHS R01-AR053645NIAMS NIH HHS R01 AR057344NIAMS NIH HHS R01-AR057344
6 · The paper itself

Abstract

objectivesAdvanced age is a factor associated with altered fracture healing. Delays in healing may increase the incidence of complications in the elderly, who are less able to tolerate long periods of immobilization and activity restrictions. This study sought to determine whether fracture repair could be enhanced in elderly animals by: (1) inhibiting macrophage activation, (2) blocking the M-CSF receptor c-fms, and (3) inhibiting monocyte trafficking using CC chemokine receptor-2 (CCR2) knockout mice.

methodsClosed unstable tibial shaft fractures were produced in mice aged 4, 12, and 78 weeks. Mice were then fed a diet containing PLX3397 or a control diet from days 1-10 after injury. Fractures were similarly made in CCR2 mice aged 78 weeks. The fracture callus was collected during fracture healing and was assessed for its size and the presence of macrophages, both of which were evaluated using the Mann-Whitney U test.

resultsPLX3397 treatment resulted in a decrease in the number of macrophages in the fracture callus at day 5. Calluses in juvenile mice trended toward being smaller compared with those in elderly mice (P = 0.08). There was also a trend toward larger callus size and increased bone formation in PLX3397-treated elderly animals when compared with those of the control animals (P = 0.12). Similar increases in bone formation (P = 0.013) and decreases in cartilage within the callus (P = 0.03) were seen at day 10 in CCR2 mice.

conclusionsThe inhibition of macrophages in elderly mice may lead to an acceleration of fracture healing. Altering macrophage activation after fracture may represent a therapeutic strategy for preventing delayed healing and nonunion in the elderly.

Indexed as

Age FactorsAnimalsFracture HealingMacrophagesMaleMiceMice, Inbred C57BLMice, KnockoutProtein Kinase InhibitorsReceptor, Macrophage Colony-Stimulating FactorReceptors, CCR2Tibial FracturesCcr2 protein, mouseProtein Kinase InhibitorsReceptor, Macrophage Colony-Stimulating FactorReceptors, CCR2

Identifiers

PMID24378434
PMCPMC3965608
OpenAlexW2041180792

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.