ArticleJournal of orthopaedic trauma2014
Intravenous application of CD271-selected mesenchymal stem cells during fracture healing.
Article in Journal of orthopaedic trauma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Timing for Intravenous Injection of Mesenchymal Stem Cells to Enhance Fracture Healing.Journal of functional biomaterials · 2026Article
- CD271 Identifies a Subpopulation with Enhanced Neural-like Potential Within Wharton Jelly Derived Mesenchymal Stem/Stromal Cells.International journal of molecular sciences · 2026Article
- Taohong Siwu Decoction Regulates MSC-Mediated H-Type Angiogenesis to Accelerate Bone Fracture Healing Through VHL/HIF-1α Ubiquitination.Mediators of inflammation · 2026Article
- Cell Surface Markers of Mesenchymal Stem Cells: Current Knowledge and Advances in Characterization Technologies.Life (Basel, Switzerland) · 2025Review
- Comparison of the effects of hydrogel and normal saline as carriers of MSC on fracture healing in a rat long bone fracture model.Journal of orthopaedic surgery and research · 2025Article
- Revolutionizing bone defect healing: the power of mesenchymal stem cells as seeds.Frontiers in bioengineering and biotechnology · 2024Review
- Optimal concentration of mesenchymal stem cells for fracture healing in a rat model with long bone fracture.World journal of stem cells · 2022Article
- Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.Regenerative medicine · 2019Article
- Clinical and Research Approaches to Treat Non-union Fracture.Current osteoporosis reports · 2018Review
- Challenges of bone tissue engineering in orthopaedic patients.World journal of orthopedics · 2017Article
- Prospective Review of Mesenchymal Stem Cells Differentiation into Osteoblasts.Orthopaedic surgery · 2017Review
- Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference.Stem cells (Dayton, Ohio) · 2016Article
- Influence of Bone and Muscle Injuries on the Osteogenic Potential of Muscle Progenitors: Contribution of Tissue Environment to Heterotopic Ossification.Stem cells translational medicine · 2016Article
- Mesenchymal Stromal Cells Implantation in Combination with Platelet Lysate Product Is Safe for Reconstruction of Human Long Bone Nonunion.Cell journal · 2016Article
- Pre-exposure of human adipose mesenchymal stem cells to soluble factors enhances their homing to brain cancer.Stem cells translational medicine · 2015Article
- Diabetes reduces mesenchymal stem cells in fracture healing through a TNFα-mediated mechanism.Diabetologia · 2015Article
- Article
- Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing.International journal of clinical and experimental pathology · 2015Article
- Utilization of Multiphoton Imaging For Real-Time Fate Determination of Mesenchymal Stem Cells in an Immunocompetent Mouse Model.Journal of stem cell research & therapy · 2014Article
- Article
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Authors and funding
7 authors.
Funding
Abstract
objectivesCirculating mesenchymal stem cells (MSCs) participate in fracture healing and can be used to enhance fracture healing. This study investigated how CD271-selected MSCs travel in circulation and when is the optimal time to apply MSCs intravenously during fracture healing.
methodsBased on the expression of CD271, MSCs were isolated from human bone marrow and labeled with cypate, a near-infrared fluorochrome. A unilateral closed fracture was created at the femur in immunodeficient mice. The cypate-labeled MSCs were injected into the tail vein of the mice at days 1 and 3 after fracture and were tracked by near-infrared imaging. The mice were euthanized at 3 weeks after fracture. Immunohistochemistry was performed to detect human MSCs at the fracture sites. Migration of CD271-selected MSCs, under the influence of stem cell-derived factor-1, was assessed in vitro.
resultsIntravenously injected at day 1, but not day 3, after fracture, CD271-selected MSCs accumulated at the fracture sites significantly and lasted for at least 7 days. All fractures, with or without MSC injections, healed in 3 weeks. Human cells were localized at the fracture sites in mice by immunohistochemistry. CD271-selected MSCs migrated toward the medium contained stem cell-derived factor-1 in vitro.
conclusionsAfter intravenous injection, CD271-selected MSCs were recruited to the fracture sites. The stages of fracture healing influenced the homing of culture-expanded MSCs. In mice, an optimal window of intravenous injection of MSCs was around 24 hours after fracture. CLINICAL RELEVANCE: Intravenous application of MSCs may serve as a practical route to deliver stem cells for the treatment of fracture nonunion and delayed union.
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