ArticleJournal of visualized experiments : JoVE2013
Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Leveraging factors that control alveolar epithelial cell fate enables large-scale expansion for lung tissue engineering.The Journal of clinical investigation · 2026Article
- Development of a 3D tumor model based on decellularized matrix using high-throughput approaches.Frontiers in bioengineering and biotechnology · 2025Review
- Bioengineering and Clinical Translation of Human Lung and its Components.Advanced biology · 2023Review
- Emerging Paradigms in Bioengineering the Lungs.Bioengineering (Basel, Switzerland) · 2022Review
- Viability of acellular biologic graft for nipple-areolar complex reconstruction in a non-human primate model.Scientific reports · 2021Article
- Implications for Extracellular Matrix Interactions With Human Lung Basal Stem Cells in Lung Development, Disease, and Airway Modeling.Frontiers in pharmacology · 2021Review
- A Two-Step Bioreactor for Decellularized Lung Epithelialization.Cells, tissues, organs · 2021Article
- Extracellular matrix remodelling in COPD.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- Acellular Biologic Nipple-Areolar Complex Graft:Tissue engineering. Part A · 2020Article
- Effect of normal saline flush injection into a bronchus on lung decellularization.Journal of thoracic disease · 2019Article
- Trans-differentiation of human adipose-derived mesenchymal stem cells into cardiomyocyte-like cells on decellularized bovine myocardial extracellular matrix-based films.Journal of materials science. Materials in medicine · 2018Article
- Canine Placenta Recellularized Using Yolk Sac Cells with Vascular Endothelial Growth Factor.BioResearch open access · 2018Article
- Lung regeneration: steps toward clinical implementation and use.Current opinion in anaesthesiology · 2017Review
- Advances in the Knowledge about Kidney Decellularization and Repopulation.Frontiers in bioengineering and biotechnology · 2017Review
- Characterization of an Acellular Scaffold for a Tissue Engineering Approach to the Nipple-Areolar Complex Reconstruction.Cells, tissues, organs · 2017Article
- Lung bioengineering: physical stimuli and stem/progenitor cell biology interplay towards biofabricating a functional organ.Respiratory research · 2016Review
- Lung Regeneration: Endogenous and Exogenous Stem Cell Mediated Therapeutic Approaches.International journal of molecular sciences · 2016Review
- Revascularization of decellularized lung scaffolds: principles and progress.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Engineering pulmonary vasculature in decellularized rat and human lungs.Nature biotechnology · 2015Article
- Design and validation of a clinical-scale bioreactor for long-term isolated lung culture.Biomaterials · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
There are an insufficient number of lungs available to meet current and future organ transplantation needs. Bioartificial tissue regeneration is an attractive alternative to classic organ transplantation. This technology utilizes an organ's natural biological extracellular matrix (ECM) as a scaffold onto which autologous or stem/progenitor cells may be seeded and cultured in such a way that facilitates regeneration of the original tissue. The natural ECM is isolated by a process called decellularization. Decellularization is accomplished by treating tissues with a series of detergents, salts, and enzymes to achieve effective removal of cellular material while leaving the ECM intact. Studies conducted utilizing decellularization and subsequent recellularization of rodent lungs demonstrated marginal success in generating pulmonary-like tissue which is capable of gas exchange in vivo. While offering essential proof-of-concept, rodent models are not directly translatable to human use. Nonhuman primates (NHP) offer a more suitable model in which to investigate the use of bioartificial organ production for eventual clinical use. The protocols for achieving complete decellularization of lungs acquired from the NHP rhesus macaque are presented. The resulting acellular lungs can be seeded with a variety of cells including mesenchymal stem cells and endothelial cells. The manuscript also describes the development of a bioreactor system in which cell-seeded macaque lungs can be cultured under conditions of mechanical stretch and strain provided by negative pressure ventilation as well as pulsatile perfusion through the vasculature; these forces are known to direct differentiation along pulmonary and endothelial lineages, respectively. Representative results of decellularization and cell seeding are provided.
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Registered trials
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