ArticlePloS one2013
A mutant Brassica napus (canola) population for the identification of new genetic diversity via TILLING and next generation sequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Haploid Mutation Mapping Identifies a Homoeologous Non-Reciprocal Translocation Linked to Reduced Fibre and Enhanced Protein in Brassica napus.Plant biotechnology journal · 2026Article
- Beyond Resistance Genes: Silencing Susceptibility.International journal of molecular sciences · 2026Review
- Genotyping-by-Sequencing Analysis Reveals Associations between Agronomic and Oil Traits in Gamma Ray-Derived Mutant Rapeseed (Plants (Basel, Switzerland) · 2024Article
- Reduced glucosinolate content in oilseed rape (Brassica napus L.) by random mutagenesis of BnMYB28 and BnCYP79F1 genes.Scientific reports · 2023Article
- Perspectives for integrated insect pest protection in oilseed rape breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Review
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- Genomics Armed With Diversity Leads the Way inFrontiers in genetics · 2021Review
- A simple and efficientPlant direct · 2020Article
- Biochemical and physiological flexibility accompanies reduced cellulose biosynthesis in BrachypodiumAoB PLANTS · 2019Article
- Manipulation of crossover frequency and distribution for plant breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Article
- FANCM Limits Meiotic Crossovers in Brassica Crops.Frontiers in plant science · 2018Article
- Article
- Mutagenesis and TILLING to Dissect Gene Function in Plants.Current genomics · 2016Article
- Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage.Frontiers in plant science · 2016Article
- Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.BMC genomics · 2015Article
- Rapid identification and recovery of ENU-induced mutations with next-generation sequencing and Paired-End Low-Error analysis.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated a Brassica napus (canola) population of 3,158 EMS-mutagenised lines and used TILLING to demonstrate that the population has a high enough mutation density that it will be useful for identification of mutations in genes of interest in this important crop species. TILLING is a reverse genetics technique that has been successfully used in many plant and animal species. Classical TILLING involves the generation of a mutagenised population, followed by screening of DNA samples using a mismatch-specific endonuclease that cleaves only those PCR products that carry a mutation. Polyacrylamide gel detection is then used to visualise the mutations in any gene of interest. We have used this TILLING technique to identify 432 unique mutations in 26 different genes in B. napus (canola cv. DH12075). This reflects a mutation density ranging from 1/56 kb to 1/308 kb (depending on the locus) with an average of 1/109 kb. We have also successfully verified the utility of next generation sequencing technology as a powerful approach for the identification of rare mutations in a population of plants, even in polyploid species such as B. napus. Most of the mutants we have identified are publically available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.