ArticlePloS one2013
Nitric oxide in plants: the roles of ascorbate and hemoglobin.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Nitric oxide as an integral element in priming-induced tolerance and plant stress memory.Journal of experimental botany · 2025Review
- Nitrate attenuates abscisic acid signaling via NIN-LIKE PROTEIN8 in Arabidopsis seed germination.The Plant cell · 2025Article
- The Key Targets of NO-Mediated Post-Translation Modification (PTM) Highlighting the Dynamic Metabolism of ROS and RNS in Peroxisomes.International journal of molecular sciences · 2024Review
- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Peroxidase activity of rice (Oryza sativa) hemoglobin: distinct role of tyrosines 112 and 151.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- Nitric Oxide Acts as an Inhibitor of Postharvest Senescence in Horticultural Products.International journal of molecular sciences · 2022Review
- Flooding Tolerance in Sweet Potato (Antioxidants (Basel, Switzerland) · 2022Article
- Characterization of Isolated Extracts fromMolecules (Basel, Switzerland) · 2020Article
- Sugar beet hemoglobins: reactions with nitric oxide and nitrite reveal differential roles for nitrogen metabolism.The Biochemical journal · 2019Article
- Seed Treatment withInternational journal of molecular sciences · 2019Article
- Elevated COProtoplasma · 2019Article
- Redox control and autoxidation of class 1, 2 and 3 phytoglobins from Arabidopsis thaliana.Scientific reports · 2018Article
- Nitric oxide participates in plant flowering repression by ascorbate.Scientific reports · 2016Article
- When Bad Guys Become Good Ones: The Key Role of Reactive Oxygen Species and Nitric Oxide in the Plant Responses to Abiotic Stress.Frontiers in plant science · 2016Review
- Differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and S-nitrosylation.Journal of experimental botany · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascorbic acid and hemoglobins have been linked to nitric oxide metabolism in plants. It has been hypothesized that ascorbic acid directly reduces plant hemoglobin in support of NO scavenging, producing nitrate and monodehydroascorbate. In this scenario, monodehydroascorbate reductase uses NADH to reduce monodehydroascorbate back to ascorbate to sustain the cycle. To test this hypothesis, rates of rice nonsymbiotic hemoglobin reduction by ascorbate were measured directly, in the presence and absence of purified rice monodehydroascorbate reductase and NADH. Solution NO scavenging was also measured methodically in the presence and absence of rice nonsymbiotic hemoglobin and monodehydroascorbate reductase, under hypoxic and normoxic conditions, in an effort to gauge the likelihood of these proteins affecting NO metabolism in plant tissues. Our results indicate that ascorbic acid slowly reduces rice nonsymbiotic hemoglobin at a rate identical to myoglobin reduction. The product of the reaction is monodehydroascorbate, which can be efficiently reduced back to ascorbate in the presence of monodehydroascorbate reductase and NADH. However, our NO scavenging results suggest that the direct reduction of plant hemoglobin by ascorbic acid is unlikely to serve as a significant factor in NO metabolism, even in the presence of monodehydroascorbate reductase. Finally, the possibility that the direct reaction of nitrite/nitrous acid and ascorbic acid produces NO was measured at various pH values mimicking hypoxic plant cells. Our results suggest that this reaction is a likely source of NO as the plant cell pH drops below 7, and as nitrite concentrations rise to mM levels during hypoxia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.