ArticleHippokratia2013
Uric acid induces caspase-1 activation, IL-1β secretion and P2X7 receptor dependent proliferation in primary human lymphocytes.
Article in Hippokratia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- The emerging role of neutrophil extracellular traps in autoimmune and autoinflammatory diseases.MedComm · 2025Review
- Association between advanced lung cancer inflammation index and acute gouty arthritis in Dalian, China: a cross-sectional study.Frontiers in nutrition · 2025Article
- Understanding the interplay between psoriatic arthritis and gout: "Psout".Rheumatology international · 2024Review
- Shirebi granules ameliorate acute gouty arthritis by inhibiting NETs-induced imbalance between immunity and inflammation.Chinese medicine · 2024Article
- Trained innate immunity as a potential link between preeclampsia and future cardiovascular disease.Frontiers in endocrinology · 2024Review
- The molecular mechanism of neutrophil extracellular traps and its role in bone and joint disease.Heliyon · 2023Review
- Physiologic roles of P2 receptors in leukocytes.Journal of leukocyte biology · 2022Review
- A novel definition and treatment of hyperinflammation in COVID-19 based on purinergic signalling.Purinergic signalling · 2022Review
- Xanthine Oxidase-Induced Inflammatory Responses in Respiratory Epithelial Cells: A Review in Immunopathology of COVID-19.International journal of inflammation · 2021Review
- At the crossroads of gout and psoriatic arthritis: "psout".Clinical rheumatology · 2020Review
- P2X7 Receptor Stimulation Is Not Required for Oxalate Crystal-Induced Kidney Injury.Scientific reports · 2019Article
- Increased expression of the P2X7 receptor in temporal lobe epilepsy: Animal models and clinical evidence.Molecular medicine reports · 2019Article
- Article
- Urate crystals directly activate the T-cell receptor complex and induce T-cell proliferation.Biomedical reports · 2017Article
- CaWorld journal of gastroenterology · 2017Article
- Asymptomatic hyperuricemia and chronic kidney disease: Narrative review of a treatment controversial.Journal of advanced research · 2017Review
- Purinergic signalling links mechanical breath profile and alveolar mechanics with the pro-inflammatory innate immune response causing ventilation-induced lung injury.Purinergic signalling · 2017Review
- Investigation into the association between P2RX7 gene polymorphisms and susceptibility to primary gout and hyperuricemia in a Chinese Han male population.Rheumatology international · 2017Article
- Correlation between P2X7 receptor gene polymorphisms and gout.Rheumatology international · 2015Review
- Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUrate through Nacht Domain, Leucine-Rich Repeat, and pyrin domain-containing protein 3 (NALP3) dependent caspase-1 activation stimulates macrophages to secrete inteleukin-1β (IL-1β). Purinergic receptor P2X7 plays a role in the urate induced NALP3 activation. Urate also enhances adaptive immunity indirectly through its effect on antigen presenting cells. In this study, the direct effect of urate on primary human lymphocytes was evaluated.
methodsLymphocytes were cultured with or without monosodium urate crystals in the presence or not of a P2X7 inhibitor. Caspase-1 activity was assessed colorimetrically in cell lysates and IL-1β was measured in supernatants with ELISA. Whole lymphocyte viability and proliferation, as well as T-cell proliferation were assessed by means of 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay and of flow cytometry respectively.
resultsUrate induced caspase-1 activation and IL-1β release by lymphocytes. It also induced proliferation of whole lymphocytes and T-cells as well. P2X7 inhibitor abrogated lymphocyte proliferation.
conclusionsUrate, a well defined danger signal, stimulates directly human lymphocytes in a P2X7 dependent way. The subsequent IL-1β secretion could enhance inflammation, whereas expansion of lymphocyte clones could facilitate a subsequent adaptive immune response.
Indexed as
Identifiers
24376319PMC3743618W2113070032What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.