ArticleJournal of experimental botany2014
Global and local perturbation of the tomato microRNA pathway by a trans-activated DICER-LIKE 1 mutant.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- Molecular characterization of cross-kingdom RNA interference inFrontiers in plant science · 2023Article
- MicroRNA162 regulates stomatal conductance in response to low night temperature stressFrontiers in plant science · 2023Article
- Article
- Tomato MicroRNAs and Their Functions.International journal of molecular sciences · 2022Review
- Knockout of SlTOM1 and SlTOM3 results in differential resistance to tobamovirus in tomato.Molecular plant pathology · 2022Article
- siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants.Scientific reports · 2020Article
- RNA silencing-related genes contribute to tolerance of infection with potato virus X and Y in a susceptible tomato plant.Virology journal · 2020Article
- Article
- DCL-suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology.Molecular plant pathology · 2019Article
- Functional Characterization of microRNA171 Family in Tomato.Plants (Basel, Switzerland) · 2019Article
- A novel DCL2-dependent miRNA pathway in tomato affects susceptibility to RNA viruses.Genes & development · 2018Article
- Redistribution of CHH Methylation and Small Interfering RNAs across the Genome of TomatoThe Plant cell · 2018Article
- TomatoHorticulture research · 2018Article
- miRCat2: accurate prediction of plant and animal microRNAs from next-generation sequencing datasets.Bioinformatics (Oxford, England) · 2017Article
- Signatures of selection and host-adapted gene expression of the Phytophthora infestans RNA silencing suppressor PSR2.Molecular plant pathology · 2017Article
- Sp-miR396a-5p acts as a stress-responsive genes regulator by conferring tolerance to abiotic stresses and susceptibility to Phytophthora nicotianae infection in transgenic tobacco.Plant cell reports · 2015Article
- Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission.Planta · 2015Article
- RNA sequencing and functional analysis implicate the regulatory role of long non-coding RNAs in tomato fruit ripening.Journal of experimental botany · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DICER-like 1 (DCL1) is a major player in microRNA (miRNA) biogenesis and accordingly, its few known loss-of-function mutants are either lethal or display arrested development. Consequently, generation of dcl1 mutants by reverse genetics and functional analysis of DCL1 in late-developing organs are challenging. Here, these challenges were resolved through the unique use of trans-activated RNA interference. Global, as well as organ-specific tomato DCL1 (SlDCL1) silencing was induced by crossing the generated responder line (OP:SlDCL1IR) with the appropriate driver line. Constitutive trans-activation knocked down SlDCL1 levels by ~95%, resulting in severe abnormalities including post-germination growth arrest accompanied by decreased miRNA and 21-nucleotide small RNA levels, but prominently elevated levels of 22-nucleotide small RNAs. The increase in the 22-nucleotide small RNAs was correlated with specific up-regulation of SlDCL2b and SlDCL2d, which are probably involved in their biogenesis. Leaf- and flower-specific OP:SlDCL1IR trans-activation inhibited blade outgrowth, induced premature bud senescence and produced pale petals, respectively, emphasizing the importance of SlDCL1-dependent small RNAs in these processes. Together, these results establish OP:SlDCL1IR as an efficient tool for analysing processes regulated by SlDCL1-mediated gene regulation in tomato.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.