ArticlePathogens and disease2014
Comparison and characterization of granulocyte cell models for Anaplasma phagocytophilum infection.
Article in Pathogens and disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Obligatory intracellular bacterium Anaplasma phagocytophilum AnkA regulates actin dynamics and spatiotemporal bacterial release.PLoS pathogens · 2026Article
- Whole transcriptome analysis reveals differential gene expression associated with Anaplasma phagocytophilum invading HL-60 cells.Parasites & vectors · 2026Article
- Comparison of Gene Editing versus a Neutrophil Elastase Inhibitor as Potential Therapies forJournal of cellular immunology · 2022Article
- Neutrophil-induced ferroptosis promotes tumor necrosis in glioblastoma progression.Nature communications · 2020Article
- What Is the Evolutionary Fingerprint in Neutrophil Granulocytes?International journal of molecular sciences · 2020Review
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- The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells.Scientific reports · 2019Article
- Alternative Splicing of Differentiated Myeloid Cell Transcripts after Infection byFrontiers in cellular and infection microbiology · 2018Article
- Interferon-γ-dependent control of Anaplasma phagocytophilum by murine neutrophil granulocytes.Parasites & vectors · 2017Article
- Genome-WideFrontiers in cellular and infection microbiology · 2016Article
- The role of CD8 T lymphocytes in rickettsial infections.Seminars in immunopathology · 2015Review
- Global DNA methylation changes and differential gene expression in Anaplasma phagocytophilum-infected human neutrophils.Clinical epigenetics · 2015Article
- Bioinformatic and mass spectrometry identification of Anaplasma phagocytophilum proteins translocated into host cell nuclei.Frontiers in microbiology · 2015Article
- Stat1 negatively regulates immune-mediated injury with Anaplasma phagocytophilum infection.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Anaplasma phagocytophilum, an obligate intracellular bacterium, modifies functions of its in vivo host, the neutrophil. The challenges of using neutrophils ex vivo necessitate cell line models. However, cell line infections do not currently mimic ex vivo neutrophil infection characteristics. To understand these discrepancies, we compared infection of cell lines to ex vivo human neutrophils and differentiated hematopoietic stem cells with regard to infection capacity, oxidative burst, host defense gene expression, and differentiation. Using established methods, marked ex vivo neutrophil infection heterogeneity was observed at 24-48 h necessitating cell sorting to obtain homogeneously infected cells at levels observed in vivo. Moreover, gene expression of infected cell lines differed markedly from the prior standard of unsorted infected neutrophils. Differentiated HL-60 cells sustained similar infection levels to neutrophils in vivo and closely mimicked functional and transcriptional changes of sorted infected neutrophils. Thus, care must be exercised using ex vivo neutrophils for A. phagocytophilum infection studies because a major determinant of transcriptional and functional changes among all cells was the intracellular bacteria quantity. Furthermore, comparisons of ex vivo neutrophils and the surrogate HL-60 cell model allowed the determination that specific cellular functions and transcriptional programs are targeted by the bacterium without significantly modifying differentiation.
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Registered trials
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