ArticleChembiochem : a European journal of chemical biology2014
A general method for artificial metalloenzyme formation through strain-promoted azide-alkyne cycloaddition.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 103 citations in OpenAlex.
- Artificial Gold Enzymes Using a Genetically Encoded Thiophenol-Based Noble-Metal-Binding Ligand.Angewandte Chemie (International ed. in English) · 2025Article
- De novo design and evolution of an artificial metathase for cytoplasmic olefin metathesis.Nature catalysis · 2025Article
- Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis.Chemical reviews · 2024Review
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- Iridium(III) polypyridine artificial metalloenzymes with tunable photophysical properties: a new platform for visible light photocatalysis in aqueous solution.Dalton transactions (Cambridge, England : 2003) · 2023Article
- Review
- Organometallic catalysis in aqueous and biological environments: harnessing the power of metal carbenes.Chemical science · 2022Review
- Controlling the optical and catalytic properties of artificial metalloenzyme photocatalysts using chemogenetic engineering.Chemical science · 2022Article
- A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase.ACS central science · 2021Article
- Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation.Angewandte Chemie (International ed. in English) · 2021Article
- A Hydroxyquinoline-Based Unnatural Amino Acid for the Design of Novel Artificial Metalloenzymes.Chembiochem : a European journal of chemical biology · 2020Article
- Unlocking the therapeutic potential of artificial metalloenzymes.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- Site-selective nitrenoid insertions utilizing postfunctionalized bifunctional rhodium(ii) catalysts.Chemical science · 2019Article
- Chemoselective Cyclopropanation over Carbene Y-H Insertion Catalyzed by an Engineered Carbene Transferase.The Journal of organic chemistry · 2018Article
- Evolving artificial metalloenzymes via random mutagenesis.Nature chemistry · 2018Article
- Selective C-H bond functionalization using repurposed or artificial metalloenzymes.Current opinion in chemical biology · 2017Review
- Design and engineering of artificial oxygen-activating metalloenzymes.Chemical Society reviews · 2016Review
- Repurposing the translation apparatus for synthetic biology.Current opinion in chemical biology · 2015Review
- Preparation, Characterization, and Oxygenase Activity of a Photocatalytic Artificial Enzyme.Chembiochem : a European journal of chemical biology · 2015Article
- Engineering a dirhodium artificial metalloenzyme for selective olefin cyclopropanation.Nature communications · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Strain-promoted azide-alkyne cycloaddition (SPAAC) can be used to generate artificial metalloenzymes (ArMs) from scaffold proteins containing a p-azido-L-phenylalanine (Az) residue and catalytically active bicyclononyne-substituted metal complexes. The high efficiency of this reaction allows rapid ArM formation when using Az residues within the scaffold protein in the presence of cysteine residues or various reactive components of cellular lysate. In general, cofactor-based ArM formation allows the use of any desired metal complex to build unique inorganic protein materials. SPAAC covalent linkage further decouples the native function of the scaffold from the installation process because it is not affected by native amino acid residues; as long as an Az residue can be incorporated, an ArM can be generated. We have demonstrated the scope of this method with respect to both the scaffold and cofactor components and established that the dirhodium ArMs generated can catalyze the decomposition of diazo compounds and both Si-H and olefin insertion reactions involving these carbene precursors.
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