ArticleJournal of clinical laboratory analysis2014
Issues in identifying germ tube positive yeasts by conventional methods.
Article in Journal of clinical laboratory analysis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Article
- HWP1 Gene Sequence Diversity and Antifungal Susceptibility Patterns in Colombian Vulvovaginal Isolates of theInternational journal of microbiology · 2025Article
- Lack of Candida africana in Ugandan pregnant women: results from a pilot study using MALDI-ToF.BMC research notes · 2024Article
- A Simple, Fast, and Reliable Method for the Identification ofEnvironmental health insights · 2024Article
- Biochemical typing and evaluation of pathogenicity in vulvovaginal isolates of Candida albicans complexBiomedica : revista del Instituto Nacional de Salud · 2023Article
- Prevalence of Closely RelatedPolish journal of microbiology · 2023Article
- A Review of Studies onAdvanced biomedical research · 2022Review
- Article
- Differentiation ofCurrent medical mycology · 2021Article
- Molecular Characterization of Highly Susceptible Candida africana from Vulvovaginal Candidiasis.Mycopathologia · 2015Article
- Molecular Characterization of Candida africana in Genital Specimens in Shanghai, China.BioMed research international · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida speciation is vital for epidemiology and management of candidiasis. Nonmolecular conventional methods often fail to identify closely related germ tube positive yeasts from clinical specimens. The present study was conducted to identify these yeasts and to highlight issues in conventional versus molecular methods of identification. A total of 98 germ tube positive yeasts from high vaginal swabs were studied over a 12-month period. Isolates were examined with various methods including growth at 42 °C and 45 °C on Sabouraud dextrose agar (SDA), color development on CHROMagar Candida medium, chlamydospore production on corn meal agar at 25 °C, carbohydrate assimilation using ID 32C system, and polymerase chain reaction using a single pair of primers targeting the hyphal wall protein 1 (Hwp1) gene. Of all the isolates studied, 97 were molecularly confirmed as C. albicans and one isolate was identified as C. dubliniensis. No C. africana was detected in this study. The molecular method used in our study was an accurate and useful tool for discriminating C. albicans, C. dubliniensis, and C. africana. The conventional methods, however, were less accurate and riddled with many issues that will be discussed in further details.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.