ArticlePflugers Archiv : European journal of physiology1987
Single glutamate-gated synaptic channels at the crayfish neuromuscular junction. I. The effect of enzyme treatment.
Article in Pflugers Archiv : European journal of physiology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 26 citations in OpenAlex.
- Depolarization increases the single-channel conductance and the open probability of crayfish glutamate channels.Biophysical journal · 1998Article
- Activation kinetics of glutamate receptor channels from wild-type Drosophila muscle.Pflugers Archiv : European journal of physiology · 1996Article
- How do patch clamp seals form? A lipid bleb model.Pflugers Archiv : European journal of physiology · 1990Article
- Glutamate receptor-channel gating. Maximum likelihood analysis of gigaohm seal recordings from locust muscle.Biophysical journal · 1990Article
- Characteristics of Na+ channels and Cl- conductance in resealed muscle fibre segments from patients with myotonic dystrophy.The Journal of physiology · 1990Article
- Rapid activation, desensitization, and resensitization of synaptic channels of crayfish muscle after glutamate pulses.Biophysical journal · 1990Article
- Argiopine blocks glutamate-activated single-channel currents on crayfish muscle by two mechanisms.The Journal of physiology · 1989Article
- Spontaneous and evoked quantal neurotransmitter release at the neuromuscular junction of the larval housefly, Musca domestica.Pflugers Archiv : European journal of physiology · 1989Article
- Calcium dependent gating of the L-glutamate activated, excitatory synaptic channel on crayfish muscle.Pflugers Archiv : European journal of physiology · 1988Article
- Ionic permeabilities of L-glutamate activated, excitatory synaptic channel in crayfish muscle.Pflugers Archiv : European journal of physiology · 1988Article
- Glutamate receptor channel kinetics: the effect of glutamate concentration.Biophysical journal · 1988Article
- Acetylcholine activates a chloride channel as well as glutamate and GABA.Journal of comparative physiology. A, Sensory, neural, and behavioral physiology · 1988Article
- Single glutamate-gated synaptic channels at the crayfish neuromuscular junction. II. Dependence of channel open time on glutamate concentration.Pflugers Archiv : European journal of physiology · 1987Article
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2 authors.
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Abstract
Single glutamate activated ionic channels were recorded with the patch clamp technique from untreated crayfish muscle fibres with M omega seals, and after treatment with collagenase, with G omega seals. In regions with single channel activity spontaneous synaptic currents could also be recorded, and the channels were therefore identified as synaptic. The single channel current amplitude was -7 to -8 pA at the resting potential of -70 mV, representing a conductance of 100 pS. The amplitudes decreased by a factor of two when the temperature was lowered by 10 degrees C. Openings occurred in bursts, and the mean burst length varied between 0.3 ms (50 microM glutamate in the pipette) and 0.8 ms (1 mM glutamate in the pipette). After treatment with collagenase, G omega seals could be formed. The conductance of the channel and the mean burst length was not affected by the enzyme, but after treatment active spots could be found easier and they were distributed more uniformly along the fibre. After treatment the concentrations of glutamate necessary to elicit channel openings were higher (100 microM compared to 20-50 microM) and simultaneous openings of two or more channels were observed very rarely. Synaptic currents could not be recorded from preparations cleaned by collagenase (2 mg/ml) for longer than 60 min.
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