ArticleBiotechnology and bioengineering2014
Cell cultivation under different gravitational loads using a novel random positioning incubator.
Article in Biotechnology and bioengineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Astroimmunology: the effects of spaceflight and its associated stressors on the immune system.Nature reviews. Immunology · 2026Review
- Hardware-independent control for partial gravity simulation using a 2-DOF robotic device.Scientific reports · 2026Article
- Comparison of clinostat control strategies to achieve simulated microgravity with uniform gravity vector distribution.NPJ microgravity · 2026Article
- Microgravity and cancer cells: from molecular mechanisms to therapeutic strategies.Cell death discovery · 2026Review
- Tissue chips as headway model and incitement technology.Synthetic and systems biotechnology · 2025Review
- Exploring the effects of simulated microgravity on esophageal cancer cells: insights into morphological, growth behavior, adhesion, and genetic damage.Journal of biological physics · 2024Article
- The Formation of Stable Lung Tumor Spheroids during Random Positioning Involves Increased Estrogen Sensitivity.Biomolecules · 2024Article
- Simulated lunar microgravity transiently arrests growth and induces osteocyte-chondrocyte lineage differentiation in human Wharton's jelly stem cells.NPJ microgravity · 2024Article
- Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning.Cancers · 2023Article
- Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications.Micromachines · 2022Review
- Impairment of 7F2 osteoblast function by simulated partial gravity in a Random Positioning Machine.NPJ microgravity · 2022Article
- Three-Dimensional Growth of Prostate Cancer Cells Exposed to Simulated Microgravity.Frontiers in cell and developmental biology · 2022Article
- Nutraceuticals Synergistically Promote Osteogenesis in Cultured 7F2 Osteoblasts and Mitigate Inhibition of Differentiation and Maturation in Simulated Microgravity.International journal of molecular sciences · 2021Article
- Plant Gravitropism and Signal Conversion under a Stress Environment of Altered Gravity.International journal of molecular sciences · 2021Review
- Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal Proteins.International journal of molecular sciences · 2021Article
- Microgravity, Stem Cells, and Cancer: A New Hope for Cancer Treatment.Stem cells international · 2021Review
- Simulated microgravity reduces proliferation and reorganizes the cytoskeleton of human umbilical cord mesenchymal stem cells.Physiological research · 2020Article
- The effects of microgravity on differentiation and cell growth in stem cells and cancer stem cells.Stem cells translational medicine · 2020Article
- Article
- Magnetic levitational bioassembly of 3D tissue construct in space.Science advances · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Important in biotechnology is the establishment of cell culture methods that reflect the in vivo situation accurately. One approach for reaching this goal is through 3D cell cultivation that mimics tissue or organ structures and functions. We present here a newly designed and constructed random positioning incubator (RPI) that enables 3D cell culture in simulated microgravity (0 g). In addition to growing cells in a weightlessness-like environment, our RPI enables long-duration cell cultivation under various gravitational loads, ranging from close to 0 g to almost 1 g. This allows the study of the mechanotransductional process of cells involved in the conversion of physical forces to an appropriate biochemical response. Gravity is a type of physical force with profound developmental implications in cellular systems as it modulates the resulting signaling cascades as a consequence of mechanical loading. The experiments presented here were conducted on mouse skeletal myoblasts and human lymphocytes, two types of cells that have been shown in the past to be particularly sensitive to changes in gravity. Our novel RPI will expand the horizon at which mechanobiological experiments are conducted. The scientific data gathered may not only improve the sustainment of human life in space, but also lead to the design of alternative countermeasures against diseases related to impaired mechanosensation and downstream signaling processes on earth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.