ArticleApplied and environmental microbiology2014
Use of quantitative real-time PCR for direct detection of serratia marcescens in marine and other aquatic environments.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
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- The Route of Infection Influences the Contribution of Key Immunity Genes to Antibacterial Defense in Anopheles gambiae.Journal of innate immunity · 2021Article
- Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal.AMB Express · 2020Article
- Biosynthesis and Bioactivity of Prodiginine Analogs in Marine Bacteria,Frontiers in microbiology · 2019Review
- Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.Scientific reports · 2018Article
- Rapid Detection of Serratia fonticola by TaqMan Quantitative Real-Time PCR Using Primers Targeting the gyrB Gene.Current microbiology · 2017Article
- Pathogen-induced maternal effects result in enhanced immune responsiveness across generations.Ecology and evolution · 2017Article
- Analysis of the genomic sequences and metabolites of Serratia surfactantfaciens sp. nov. YD25BMC genomics · 2016Article
- Complementary approaches to diagnosing marine diseases: a union of the modern and the classic.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Systematic Analysis of White Pox Disease in Acropora palmata of the Florida Keys and Role of Serratia marcescens.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Serratia marcescens is the etiological agent of acroporid serratiosis, a distinct form of white pox disease in the threatened coral Acropora palmata. The pathogen is commonly found in untreated human waste in the Florida Keys, which may contaminate both nearshore and offshore waters. Currently there is no direct method for detection of this bacterium in the aquatic or reef environment, and culture-based techniques may underestimate its abundance in marine waters. A quantitative real-time PCR assay was developed to detect S. marcescens directly from environmental samples, including marine water, coral mucus, sponge tissue, and wastewater. The assay targeted the luxS gene and was able to distinguish S. marcescens from other Serratia species with a reliable quantitative limit of detection of 10 cell equivalents (CE) per reaction. The method could routinely discern the presence of S. marcescens for as few as 3 CE per reaction, but it could not be reliably quantified at this level. The assay detected environmental S. marcescens in complex sewage influent samples at up to 761 CE ml(-1) and in septic system-impacted residential canals in the Florida Keys at up to 4.1 CE ml(-1). This detection assay provided rapid quantitative abilities and good sensitivity and specificity, which should offer an important tool for monitoring this ubiquitous pathogen that can potentially impact both human health and coral health.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.