ArticleApplied and environmental microbiology2014
Production of lycopene in the non-carotenoid-producing yeast Yarrowia lipolytica.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 215 citations in OpenAlex.
- Research progress on metabolic engineering for efficient squalene synthesis in yeast cell factories.Biotechnology letters · 2026Review
- Characterisation of metabolic burden in Pseudomonas putida reveals precursor limitation in heterologous lycopene production.Microbial cell factories · 2026Article
- Intron location and sequence modulate gene expression in Yarrowia lipolytica.Nucleic acids research · 2026Article
- Identifying new targets for improving terpenoid biosynthesis in Yarrowia lipolytica through random genomic cytosine base editing.Microbial cell factories · 2025Article
- Efficient biosynthesis of β-caryophyllene by engineered Yarrowia lipolytica.Microbial cell factories · 2025Article
- Screening of novel β-carotene hydroxylases for the production of β-cryptoxanthin and zeaxanthin and the impact of enzyme localization and crowding on their production in Yarrowia lipolytica.Microbial cell factories · 2024Article
- Transcending membrane barriers: advances in membrane engineering to enhance the production capacity of microbial cell factories.Microbial cell factories · 2024Review
- 'Mother(Nature) knows best' - hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database.Microbial cell factories · 2024Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- Advances in the Discovery and Engineering of Gene Targets for Carotenoid Biosynthesis in Recombinant Strains.Biomolecules · 2023Review
- Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica.Bioresources and bioprocessing · 2023Article
- Characterization of an efficient CRISPR-iCas9 system in Yarrowia lipolytica for the biosynthesis of carotenoids.Applied microbiology and biotechnology · 2023Article
- Abolishing storage lipids induces protein misfolding and stress responses in Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2023Article
- Enhanced β-carotene production in Yarrowia lipolytica through the metabolic and fermentation engineering.Journal of industrial microbiology & biotechnology · 2023Article
- Engineering ofMetabolic engineering communications · 2022Review
- Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis inSynthetic and systems biotechnology · 2022Article
- Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.Synthetic and systems biotechnology · 2022Review
- Compartmentalization and transporter engineering strategies for terpenoid synthesis.Microbial cell factories · 2022Review
- Production of abscisic acid in the oleaginous yeast Yarrowia lipolytica.FEMS yeast research · 2022Article
- Carotenoids and Their Biosynthesis in Fungi.Molecules (Basel, Switzerland) · 2022Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica, GGS1 and HMG1, were overexpressed to increase the production of lycopene. All of the genes were also expressed in a Y. lipolytica strain with POX1 to POX6 and GUT2 deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene production. Lycopene is located mainly within lipid bodies, and increased lipid body formation leads to an increase in the lycopene storage capacity of Y. lipolytica. Growth-limiting conditions increase the specific lycopene content. Finally, a yield of 16 mg g(-1) (dry cell weight) was reached in fed-batch cultures, which is the highest value reported so far for a eukaryotic host.
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