ArticleMolecular cell2014
Determinants of heterochromatic siRNA biogenesis and function.
Article in Molecular cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 68 citations in OpenAlex.
- PolyAseqTrap: a universal tool for genome-wide identification and quantification of polyadenylation sites from different 3' end sequencing data.Genome biology · 2026Article
- Targeting tumor immune evasion: the role of PD-L1 siRNA in advancing cancer immunotherapy.Medical oncology (Northwood, London, England) · 2025Review
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Article
- Regulation of small RNA-mediated high temperature stress responses in crop plants.Plant cell reports · 2022Review
- Review
- A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin.Molecular cell · 2021Article
- An essential role for the piRNA pathway in regulating the ribosomal RNA pool in C. elegans.Developmental cell · 2021Article
- An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing.PLoS genetics · 2021Article
- A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin.eLife · 2020Article
- Unprogrammed epigenetic variation mediated by stochastic formation of ectopic heterochromatin.Current genetics · 2020Review
- The euchromatic histone mark H3K36me3 preserves heterochromatin through sequestration of an acetyltransferase complex in fission yeast.Microbial cell (Graz, Austria) · 2020Article
- Review
- An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.RNA biology · 2019Article
- Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.Nature communications · 2019Article
- Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly.Genes & development · 2018Article
- Bases of antisense lncRNA-associated regulation of gene expression in fission yeast.PLoS genetics · 2018Article
- Article
- Ten principles of heterochromatin formation and function.Nature reviews. Molecular cell biology · 2018Review
- Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Endogenous small interfering RNAs (siRNAs) and other classes of small RNA provide the specificity signals for silencing of transposons and repeated DNA elements at the posttranscriptional and transcriptional levels. However, the determinants that define an siRNA-producing region or control the silencing function of siRNAs are poorly understood. Here we show that convergent antisense transcription and availability of the Dicer ribonuclease are the key determinants for primary siRNA generation. Surprisingly, Dicer makes dual contributions to heterochromatin formation, promoting histone H3 lysine 9 methylation independently of its catalytic activity, in addition to its well-known role in catalyzing siRNA generation. Furthermore, sequences in the 3' UTR of an mRNA-coding gene inhibit the ability of siRNAs to promote heterochromatin formation, providing another layer of control that prevents the silencing of protein-coding RNAs. Our results reveal distinct mechanisms that limit siRNA generation to centromeric DNA repeats and prevent spurious siRNA-mediated silencing at euchromatic loci.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.