Evidence map›Paper›PMID 24373999›Full record

ArticleAnalytical biochemistry2014

High-performance liquid chromatography purification of chemically modified RNA aptamers.

Chi-Yen Lin, Zhen Huang, William Jaremko, Li Niu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chi-Yen LinDepartment of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
Zhen HuangDepartment of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
William JaremkoDepartment of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA.
Li NiuDepartment of Chemistry, Center for Neuroscience Research, University at Albany, State University of New York (SUNY), Albany, NY 12222, USA. Electronic address: lniu@albany.edu.

Funding

Mechanistic studies of the GYKI CompoundsR01NS060812 · NINDS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI NIU, LI · 2009 to 2013
$1.5M
NINDS NIH HHS R01 NS060812
6 · The paper itself

Abstract

2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.

Indexed as

Aptamers, NucleotideChromatography, High Pressure LiquidChromatography, Reverse-PhaseHalogenationIonsAptamers, NucleotideIonsChemically modified RNAHPLCRNA purification and separation

Identifiers

PMID24373999
PMCPMC5577503

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.