ArticleStructure (London, England : 1993)2014
Structure and Ca²⁺-binding properties of the tandem C₂ domains of E-Syt2.
Article in Structure (London, England : 1993), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Quantitative FRET FLIM imaging reveals multiple interactions between proteins of the ESYT, ORP, and VAP families at the endoplasmic reticulum membrane.The Journal of biological chemistry · 2026Article
- Ca2+ binding to Esyt modulates membrane contact site density in Drosophila photoreceptors.The Journal of cell biology · 2025Article
- The Extended Synaptotagmins ofPlants (Basel, Switzerland) · 2025Review
- Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress.Plant, cell & environment · 2025Article
- Stepwise membrane binding of extended synaptotagmins revealed by optical tweezers.Nature chemical biology · 2022Article
- The structure and flexibility analysis of theLife science alliance · 2021Article
- Structural studies of the shortest extended synaptotagmin with only two C2 domains fromiScience · 2021Article
- Means of intracellular communication: touching, kissing, fusing.Microbial cell (Graz, Austria) · 2021Article
- Geometry and cellular function of organelle membrane interfaces.Plant physiology · 2021Review
- Endoplasmic Reticulum-Plasma Membrane Contact Sites: Regulators, Mechanisms, and Physiological Functions.Frontiers in cell and developmental biology · 2021Review
- Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS.Acta crystallographica. Section D, Structural biology · 2019Article
- Differentiation of human adipose-derived stem cells into neuron/motoneuron-like cells for cell replacement therapy of spinal cord injury.Cell death & disease · 2019Article
- A programmable DNA-origami platform for studying lipid transfer between bilayers.Nature chemical biology · 2019Article
- Here, there, and everywhere: The importance of ER membrane contact sites.Science (New York, N.Y.) · 2018Review
- CaThe EMBO journal · 2018Article
- Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.BMC biology · 2017Article
- Article
- The Extended-Synaptotagmins.Biochimica et biophysica acta. Molecular cell research · 2017Review
- Pull-down combined with proteomic strategy reveals functional diversity of synaptotagmin I.PeerJ · 2017Article
- SMP-domain proteins at membrane contact sites: Structure and function.Biochimica et biophysica acta · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C₂ domains. One of the tandem C₂ domains of E-Syt2 is predicted to bind Ca²⁺, but no Ca²⁺-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C₂ domains of E-Syt2 in the absence and presence of Ca²⁺ and analyzed their Ca²⁺-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C₂ domains that is not substantially altered by Ca²⁺. The E-Syt2 C2A domain binds up to four Ca²⁺ ions, whereas the C₂B domain does not bind Ca²⁺. These results suggest that E-Syt2 performs an as yet unidentified Ca²⁺-dependent function through its C₂A domain and uncover fundamental differences between the properties of the tandem C₂ domains of E-Syts and synaptotagmins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.