ArticleBMC microbiology2013
The murine lung microbiome in relation to the intestinal and vaginal bacterial communities.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Article
- Effects of Host-Specific Multi-Lactic Acid Bacterial Probiotics on Performance, Carcass Traits, Meat Quality, and Gut Microbiome in Fattening Pigs.Veterinary sciences · 2026Article
- Dietary Iron Intake Impacts the Microbial Composition of the Murine Intestinal and Lung Microbiome.Nutrients · 2025Article
- Influence of host-specific and locally isolated multi-strain probiotics on piglet performance, mortality, inflammatory response, and gut microbiome.Animal bioscience · 2025Article
- Locally administered nanosuspension increases delivery of estradiol for the treatment of vaginal atrophy in mice.Drug delivery and translational research · 2025Article
- The Bacterial Genus Ramlibacter: Betaproteobacteria Capable of Surviving in Oligotrophic Environments Thanks to Several Shared Genetic Adaptation Traits.Environmental microbiology · 2025Review
- Use of Subtherapeutic Tylvalosin AgainstTransboundary and emerging diseases · 2025Article
- The effects of different lung parts, age, and batches on the lung microbiota of healthy rats.Annals of medicine · 2024Article
- Article
- Intact lung tissue and bronchoalveolar lavage fluid are both suitable for the evaluation of murine lung microbiome in acute lung injury.Microbiome · 2024Article
- What we need to know about the germ-free animal models.AIMS microbiology · 2024Review
- Sex-specific alterations in the gut and lung microbiome of allergen-induced mice.Frontiers in allergy · 2024Article
- Genome-wide mapping of gene-microbe interactions in the murine lung microbiota based on quantitative microbial profiling.Animal microbiome · 2023Article
- Nonoptimal bacteria species induce neutrophil-driven inflammation and barrier disruption in the female genital tract.Mucosal immunology · 2023Article
- Article
- The impact of bacterial exposure in early life on lung surfactant gene expression, function and respiratory rate in germ-free mice.Frontiers in microbiomes · 2023Article
- Enhancing Immunity and Modulating Vaginal Microflora Against Candidal Vaginitis Through Nanoemulsion Supplemented withInternational journal of nanomedicine · 2023Article
- Microbiota of the Pregnant Mouse: Characterization of the Bacterial Communities in the Oral Cavity, Lung, Intestine, and Vagina through Culture and DNA Sequencing.Microbiology spectrum · 2022Article
- Dynamic alterations in the lung microbiota in a rat model of lipopolysaccharide-induced acute lung injury.Scientific reports · 2022Article
- Obesity Reshapes the Microbial Population Structure along the Gut-Liver-Lung Axis in Mice.Biomedicines · 2022Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis work provides the first description of the bacterial population of the lung microbiota in mice. The aim of this study was to examine the lung microbiome in mice, the most used animal model for inflammatory lung diseases such as COPD, cystic fibrosis and asthma.Bacterial communities from broncho-alveolar lavage fluids and lung tissue were compared to samples taken from fecal matter (caecum) and vaginal lavage fluid from female BALB/cJ mice.
resultsUsing a customized 16S rRNA sequencing protocol amplifying the V3-V4 region our study shows that the mice have a lung microbiome that cluster separately from mouse intestinal microbiome (caecum). The mouse lung microbiome is dominated by Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Cyanobacteria overlapping the vaginal microbiome. We also show that removal of host tissue or cells from lung fluid during the DNA extraction step has an impact on the resulting bacterial community profile. Sample preparation needs to be considered when choosing an extraction method and interpreting data.
conclusionsWe have consistently amplified bacterial DNA from mouse lungs that is distinct from the intestinal microbiome in these mice. The gut microbiome has been extensively studied for its links to development of disease. Here we suggest that also the lung microbiome could be important in relation to inflammatory lung diseases. Further research is needed to understand the contribution of the lung microbiome and the gut-lung axis to the development of lung diseases such as COPD and asthma.
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