ArticleBMC research notes2013
Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study.
Article in BMC research notes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 78 citations in OpenAlex.
- Establishing a competence model in discriminatingBiomedical reports · 2026Article
- Genomic population structure, antimicrobial susceptibility, and clinical features ofJournal of clinical microbiology · 2026Article
- Phenotypic drug resistance and genome sequencing based identified mutations linked to resistance in Mycobacterium tuberculosis isolated from extrapulmonary clinical specimens.Scientific reports · 2026Article
- Structure based discovery of VMP-7 as a promising GlgE targeting lead againstFrontiers in microbiology · 2026Article
- Whole genome sequencing-based detection of extensively drug-resistant tuberculosis from Ethiopia.Communications medicine · 2025Article
- Global lineages of non-tuberculous mycobacteria in residential water samples from Germany.BMC microbiology · 2025Article
- Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow.Journal of visualized experiments : JoVE · 2025Article
- Linking genetic and phenotypic bedaquiline resistance in Mycobacterium tuberculosis strains from Georgia.PloS one · 2025Article
- Genotypic drug resistance and transmission clusters of Mycobacterium tuberculosis isolates among Ethiopian returnees from Saudi Arabia.PloS one · 2025Article
- Clinical and genomic features of Mycobacterium avium complex: a multi-national European study.Genome medicine · 2024Article
- First Indonesian report of WGS-based MTBC L3 discovery.BMC research notes · 2024Article
- Article
- Article
- Whole-genome sequencing-based genetic diversity, transmission dynamics, and drug-resistant mutations inFrontiers in public health · 2024Article
- Identification and specificity validation of unique and antimicrobial resistance genes to trace suspected pathogenic AMR bacteria and to monitor the development of AMR in non-AMR strains in the environment and clinical settings.Saudi journal of biological sciences · 2023Article
- Molecular Characterisation ofPathogens (Basel, Switzerland) · 2023Article
- Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction.Sensors (Basel, Switzerland) · 2023Article
- Occurrence ofAnimals : an open access journal from MDPI · 2023Article
- Phylogenetic relationships ofFrontiers in cellular and infection microbiology · 2023Article
- Divergent proinflammatory immune responses associated with the differential susceptibility of cattle breeds to tuberculosis.Frontiers in immunology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDevelopments in molecular detection and strain differentiation of members of Mycobacterium tuberculosis complex have proved to be useful. The DNA extraction method influences the amplification efficiency, causing interference on the sensitivity and respective inhibitors. The aim of this study was to standardize a simple and fast DNA extraction method, providing DNA amplification by IS6110-PCR effectively free from undue interferences.
findingsThe efficiency of the six different protocols tested in M. tuberculosis cultures has varied from 75% to 92.5%. This preliminary study evaluating the IS6110 PCR sensitivity and specificity was developed in DNA extracted from microscope slides, and achieved 100% of efficiency.
conclusionsDNA extraction by Chelex + NP-40 method from both, cultures of M. tuberculosis and smear slides, resulted in good quantity of interference free DNA, especially in samples with low concentrations of genetic material; therefore, such technique may be used for the molecular diagnosis of tuberculosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.